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We narrowed to 154 results for: hwa

Showing: 41 - 60 of 154 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...etc., microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor...isolated afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs...testing any number in the Level 2 assembly. These pathways can require a lot of optimization - which is why...

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...cell viability and increase protein production (Schwarz et. al., 2020). Harvest and purification At its....2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, ...Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau ...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to restore the DNA after... S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing...coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:. https://doi.org/10.1038...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023) for a review of other targeted...to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...breaks into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion...researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more CRISPR tools for C.... . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

  6. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...transversion editors rely on a multi-step cellular repair pathway that is known for sometimes causing double-strand...

  7. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin... M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench, J. G., LaFleur, M...

  8. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... will be able to better choose which genes and pathways to target in future small molecule screens. He...coworkers to test inhibitors of a variety of signaling pathways for their effects on the disease. This work will...

  9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... in organoid cultures are stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This...Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  10. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...trajectories and identify both up- and down-regulated pathways. Individual cellular fates were then matched with...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway...networks, and selectively activating signaling pathways. Many scientists came to the Addgene booth to ...

  13. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...To control for protein degradation due to other pathways, it is standard practice to compare degradation...ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active form of...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...earning their PhDs (3), showing that the ‘standard’ pathway into academia may in fact be just another ‘alternative...recently launched a survey “Identifying Career Pathways for PhDs in Science”, through which she collects...

  16. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...further, his lab is now studying how Argonaute pathways may be involved in preventing silencing of self-genes...self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive...

  18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... using the endogenous homology directed repair pathway, a new user-defined DNA sequence (like GFP) can...previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a...

  19. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein might...undergraduate research experiences (CUREs) as a pathway to diversify science. Evolution and Development...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies and relies...A, A>G, and T>C) installation by base editors. Pathways that compete with the desired outcome are not ...
Showing: 41 - 60 of 154 results