We narrowed to 330 results for: iCre

...Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344...Behavior of Plasmid DNA. Microbiol Spectr. 2015 Apr;3(2):10.1128/microbiolspec.PLAS-0036-2014. doi: 10.1128...10.1128/microbiolspec.PLAS-0036-2014. PMID: 26104708; PMCID: PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch...the secrets of multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012...instability: the dimer catastrophe hypothesis. Mol Microbiol. 1993 Jun;8(6):1031-8. doi: 10.1111/j.1365-2958.1993...

...begins almost 50 years ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb...intercellular signaling pathways (Ernst et al., 2014). Microbial rhodopsins are encoded by opsin genes, and include...to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? Neuronal... light: when hit with the correct wavelength, microbial rhodopsins enable ions to flow across the axon... inject genetically modified viruses encoding microbial rhodopsins into specific cerebral regions. The...PubMed PMID: 31082625. Ernst, Oliver P., et al. "Microbial and animal rhodopsins: structures, functions, ...Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic tools." Cell 147.7...

...visible under both phase contrast microscopy and electron microscopy (Fig. 1b). Gas vesicle expression...inclusions in cells with GVs via phase contrast microscopy, GVs visible in cells via TEM, and purified GVs...detectable under ultrasound and phase contrast microscopy just one day after induction of expression… but...positivity, which can be observed under phase contrast microscopy and TEM (Fig. 2c), and ultrasound imaging. If...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553, 86–90 (2018...

...molecule interactions in living cells using a microscope. Historically, molecular interactions were detected...the acceptor fluorophore can be measured using microscopy techniques. FRET measurement sensitivity makes... method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching method...donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed more recently ...RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells...Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J...

... funding. Using current resources to develop microbiology and molecular biology research programs. 30:... public about dietary health. 40:17 - 44:14 : Microbiome research - working with bacterial communities...- end : Kwabena's time in Boston - Developing microbiome expertise. Many thanks to Kwabena Duedu for ...Kaba Resources on Addgene.org Check out our Microbiology Pages Find Synthetc Biology Plasmids Find Molecular... microbiology...

...appreciated on an aesthetic level as well. Beautiful microscope images which traditionally languished in the ... can be found on his website http://www.microworldsphotography.com/. Another artist who spent his life...you’re a scientist who spends many hours at a microscope, you too can become an artist. Scientists moonlighting...artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old international ...and science? Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the...

... Plasmids! Light controls the activity of a microtubule-associated protein to regulate cell growth dynamics...light by modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their ...into two halves (Fig. 1): one containing the microtubule binding domain and the second containing the ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...mCherry-positive to eGFP positive cells through fluorescence microscopy or flow cytometry. Using the ACE system, the ...

... biological dark matter – large tracts of the microbial tree of life that remained unexplored. Using new...axenic (pure) laboratory cultures to study these microbes. From 16S rRNA sequencing, hot springs were known...YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical and chemical conditions...tiring. It feels a bit strange to be using a micropipette and gloves at a campsite. And it was exciting.... They used a mini-metagenomics method using microfluidic parallelization to sequence this highly complex...

...expert in microbial ecology but she became a member at BosLab because she wants to engineer microbes. We’ve...Laurenceau, and Tami Pudina have initiated a soil microbiome project. This project was inspired by previous...it, and analyze the data to characterize the microbiomes of the different samples. The goal is to not ...not only learn something about the microbial diversity in different soil conditions/locations but to engage...

...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...then view under an oil immersion using a light microscope. Gram positive bacteria will be stained blue .... Gram Stain Protocols. American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063...

...cellular exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted...from producer cells, and then to enrich the microvesicles containing AAVs (Figure 2). Since the development...vector for CD9 (a known marker molecule for microvesicles) can further improve AAV yields as CD9 is thought...structure of exosome AAVs is related to the use of microvesicles as a vehicle for drug delivery that has recently...PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as a novel gene delivery...

...Cytology and Van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam. Before you decide which... a couple of candidates. Before you buy a new microscope, it is smart to look at (and through) a couple...does not fold well or cannot be detected in a microscopy setup has zero practical brightness and is of...parameters into account, including the specs of your microscope (excitation wavelength, available emission filters...Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops, characterizes...

...instance I met one researcher studying how the microbiome can affect Alzheimer's - a connection I wouldn...“I Contain Multitudes” has me considering the microbiome more often than not). If you’re someone who’s...many images of brain regions, or sequences of microbial DNA they all seem to require some mathematical...of course, many companies selling antibodies, microscopes, and dissection tools, but there were also people...researcher using sequencing technologies to study microbiome interactions with Alzheimer's, I found others...

...Cytology and Van Leeuwenhoek Centre for Advanced microscopy, University of Amsterdam. The previous post in...Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited with very intense... diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) embedded in a ...fluorescent proteins of the same spectral class on the microscopy system and in the cell type (or tissue) that ...Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops, characterizes...

...TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the target antibody works...for his contributions to super-resolution light microscopy, comes from his recent Nature Communications ...ratiometric pH biosensors for super-resolution microscopy.” Endosomes are historically difficult to study...study in living cells using conventional light microscopy due to their small size (<250 nm), and studying...MEF cells. For further reading on fluorescent microscopy techniques, the first author of this article,...

...that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources instead...advances in probe design, fluorescent proteins and microscopy equipment have enhanced the ability of labs to...Hirata and Kiyokawa 2016), and molecular tension microscopy (MTM), which could monitor physical stresses ...-Molecule FRET Biosensors and Intravital FRET Microscopy." Biophysical Journal 111.6 (2016): 1103-1111...Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods 94 (2016): 33-42. PubMed PMID: 26210398...

...species, such as the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. ...Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria isolated from cheese. Knockdown...Silver. "Harnessing undomesticated life." Nature microbiology 4.2 (2019): 212. PubMed PMID: 30675034. Peters...diverse bacteria with Mobile-CRISPRi." Nature microbiology 4.2 (2019): 244. PubMed PMID: 30617347. PubMed.... "Tn7." Mobile DNA III. American Society of Microbiology, 2015. 647-667. PubMed PMID: 26104363. Additional...

...High-resolutions microscopy As indicated above, smFPs can be used with conventional microscopes, such as epifluorescence...epifluorescence or confocal microscopes, but they can also be used with the most cutting-edge imaging ... super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes...High-performance probes for light and electron microscopy." Nature methods 12.6 (2015): 568. PubMed PMID...

... using microscopy and advanced computational approaches (Keller et al., 2008). This microscopy-based lineage... important insights in developmental biology. Microscopy-based lineage tracing Over a period of 24 hours...pinhead mutant from the random mutagenesis screens. Microscopy-based lineage tracing can help illuminate how... embryonic development by scanned light sheet microscopy." science 322.5904 (2008): 1065-1069. PubMed ...

...Cytology and van Leeuwenhoek Centre for Advanced Microscopy (University of Amsterdam). GFP is the most popular...visualization with standard filter sets on a fluorescence microscope, and finally (iv) good toleration in fusion proteins...Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops, characterizes... lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 (2002): 913...fluorescent amino acid for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences...