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We narrowed to 148 results for: lenti

Showing: 41 - 60 of 148 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...individually label millions of mammalian cells through lentiviral infection and to monitor their abundance and ...optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later, Bhang says, “When... treatment. The library consists of a pool of lentiviral vectors with roughly 73 million semi-random, ...infection. When using the ClonTracer system, lentiviruses containing these barcodes infect the target ... Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources...

  2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...efficiency. Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9...Difficulty Very easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing...: Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome repair by...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...Library. This complex second-generation CRISPR lentiviral library targets nearly all human protein-coding...from the CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...

  5. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ... those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and... pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO ...-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs ...

  6. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery...toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression... Cas9. Recently, Baisong Lu’s lab developed a lentiviral capsid-based bionanoparticle system for packaging... aptamer binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used...

  8. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once thawed, there was... 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check... lentivirus...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...crux of the system is a set of heat-inducible lentiviral CAR constructs, the most basic of which is depicted..., inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the...

  11. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ... CRISPR pooled libraries have been created in lentiviral systems for mammalian screens, which enables ...However, some cell types are poorly transduced by lentivirus, and thus screening with available CRISPR libraries...Primary murine T cells cannot be transduced by lentivirus and have been difficult to manipulate genetically...for screening of cells that are refractory to lentiviral transduction, now enabling forward genetic screens...

  12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout...that you review the steps for how to use pooled lentiviral CRISPR libraries and keep the following in mind... sequencing technology. These libraries are lentiviral in nature, so please ensure that you are equipped...equipped and authorized to make and use lentivirus. Also, note that due to the size of the backbone, transformations...

  13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited...19 pandemic, the Krogan lab at UCSF generated lentiviral vectors expressing SARS-CoV-2 open reading frames...all but one are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for...(2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...parentheses.  In this example I wanted to find a lentiviral plasmid to express ubiquitin under control of...300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed...this filter will allow to refine by serotype or lentiviral prep Requests: Only see plasmids with more than... cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker ...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...purpose. For mammalian lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1)...Gateway cloning to generate our desired constructs: lentiviral expression of the human KRAS gene in mammalian...available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems (MuLE) Kit, the MultiSite ...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...their options for tools such as empty backbones, lentiviral packaging systems, and luciferase expression.... Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus...a Liquid Bacteria Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...within the lentiviral 3’ long terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration...license. In this iteration, the size of the lentiviral insert was minimized to reduce recombination ...III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These optimizations...

  18. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... NEB Stable   For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...JM109-derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...Derived from HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from ...

  20. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...available in two vector formats: all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA...gRNA from this single vector) and guide-only (lentiGuide expressing only an gRNA, for use with cells that...at Addgene, you can also order the individual lentiGuide gRNA plasmids targeting the human kinome. These...
Showing: 41 - 60 of 148 results