...favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping...plants. If your cultures have been incubated at stationary phase for too long, the cells in your culture...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...

...organismal systems described at this meeting were extraordinarily diverse. Plans to develop tools to study their...electric organs arose in different branches of the evolutionary tree at least 6-7 times and on at least 2 separate... Milkweed plants are known to many as the only Monarch butterfly food. They have chemical pathways generating...

...Crystal structures of CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide...CRBNmidi on its own, with drug ligands, and in ternary complexes with other proteins, establishing CRBNmidi...low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging...

...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

...Nobody EVER complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts...proper consideration. 3. Present an easy to follow seminar See item 2. Attendees will include grad students...

...to detect the presence or absence of a protein (Renart et al., 1979, Towbin et al., 1979, Burnett et al...from Addgene that you can make on your own.  To narrow your search, first pay attention to the validated...–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins...

...Other tools are designed to be activated within a narrower band of wavelengths to allow multiple tools, such... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...

...communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists...Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation in Science...

...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

...series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and Biomedical...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives... things up with a combination of shorter, more narrowly focused lectures, interactive panels, and a student...

...acids you can change the specificity,” explained Zhanar Abil of the University of Illinois at Urbana-Champaign...updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins...

... we learn about some of their events - science seminars, science cafes, and the SITN Blog (other programs...science in a social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - ...

...labs and the importance of the strong, cross-disciplinary collaboration they shared to the work they did...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...

...than intensely competitive. Be curious and interdisciplinary.   3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people...

...aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter, Mary O’Riordan, and Victor...don’ts of that week’s piece of a manuscript. The webinars were my favorite part due to the interaction with...

...and images in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome...For more information and a link to the archived webinar, click here. We would like to thank IDT scientists...

...proteins and PAM sequences. A total of eight evolutionarily conserved CRISPR subtypes comprised of more...subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system...

...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...