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Showing: 41 - 60 of 244 results

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Renilla luciferase from the sea pansy (Renilla reniformis) emits a blue light with a peak emission at 480nm...Vargulin) luciferin None Yes Sea pansy(Renilla reniformis) 480 36 Coelenterazine None No Deep sea coceopod...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...reverse screens, except that they begin with a uniform population possessing a specific mutation or phenotype... the cellular pathway of interest conveys no significant survival advantage, such as a non-essential mechanism...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... to detect mRNA from 10 bacteria of clinical significance and four clinically relevant host biomarkers... Jennifer Tsang...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...amounts of access or feedback. One of the most significant shifts in scientific communication is the rising...research materials available to the community significantly sooner. Plus, authors can include the Addgene...

  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...

  10. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...therapies that are currently approved or have made significant progress in clinical trials.     Figure...delivery choice. In sum, CRISPR therapies have made significant clinical strides since we first wrote this blog...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...necessary step during production), there is a significant risk for aerosol contamination. As most centrifugation...sharps procedures for your organization will significantly reduce the risk of an accident (the most common...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a .../genetics/iyab006       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...10.1016/j.celrep.2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...disease, and HIV. A notable landmark study showed significant clinical efficacy against a form of blindness... of LCA and mutations in RPE65 (n=3), showed significant improvements in vision over a period of 1 to ...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... a freshly collected lentiviral prep may be significantly higher than the same prep that has been stored...PMCID: PMC1534021. 3. Zhang, Bing, et al. "The significance of controlled conditions in lentiviral vector...

  20. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...study,epigenetic changes were statistically significantly associated with reduced life-span, but no changes...controlled, the reporter consistently showed significantly different FRET levels between unmethylated and...
Showing: 41 - 60 of 244 results