We narrowed to 232 results for: pET

... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...

...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round data.... Bobae An will host a Q&A session after the competition, where you can get all your burning AAV questions...

...a relative term — even armed with the highest-competency cells you will still struggle to get transformants... or if you get a little overzealous with your pipetting. Another is the possibility of carrying over contaminants...practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification columns...buffer bottles Fewer plastic consumables Fewer pipette tips needed in workflow Reduced waste from ...

...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...a single use. So how about washing and reusing pipette tips in a high-throughput and efficient way? Grenova... devices that are compatible with a variety of pipette tips. This is part of a cultural change in how ...

... Gram-negative and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different...CRISPRi to a much wider swath of bacterial species (Peters et al., 2019). The systems differ on how exactly...10: . https://doi.org/10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, ...Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA,...

... to help them spread through bacterial genomes (Peters et al., 2017). Type I CAST systems rely on the ...CAST activity allowed certain mutated phages to outcompete their brethren, and the researchers isolated ...–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin,...

...methylation competent E. coli for this procedure to work properly. Directly transform chemically competent cells...are then transformed directly into chemically competent E. coli and transformants selected with the appropriate...

...30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse...Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just...heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively remove a stop...

...that allow their host organisms to thrive in competitive environments. The genes encoding these functions...triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only organism that... are also less aggressive ways to survive in competitive environments — like by living where no one else...

...as a device fits on the deck, and it’s easy to pipette samples in and out of it, you can set up a procedure...using electronic multichannel and single channel pipettes. These can speed up any project in which you need... to do a lot of aliquoting. Simply program the pipette with how much total sample you need and the volume...

...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...the bench and all consumables with 10% bleach.  Pipette slowly If you are using a manual droplet generator...automated droplet generator, you should avoid pipetting too quickly when making your virus dilutions. ...

...just one or two recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...(1991): 62-66. PubMed PMID: 2003926. 2. Bubeck, Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning...

...mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad’s Pattern team,...between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally...Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, ...

... samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...ensure that nothing is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at...Each sample that is transformed is plated onto a petri dish plate that is also barcoded. The barcodes on...

...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

...decreasing the probability of creating replication-competent lentivirus (RCL) in your target cells.  Q4: How...targeted. Q6: Are transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer...are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential for...

...your experiments. Don’t dive directly into the pipetting! MoClo nomenclature and structure can be quite...To combat this, you can increase the volume of competent cells you plate after each transformation and/...high-throughput, we recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your...

...sample is immediately ready to transform into competent cells. The master mix of enzymes can be purchased...master mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically...terminator sequence), since this would directly compete with the required single-stranded annealing and...

...restriction-deficient; good strain for cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB...lacY1 proA2 rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning and storage of unstable ...XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids, ligated...

...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...