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We narrowed to 231 results for: pET

Showing: 41 - 60 of 231 results

  1. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...

  2. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round data.... Bobae An will host a Q&A session after the competition, where you can get all your burning AAV questions...

  3. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...a relative term — even armed with the highest-competency cells you will still struggle to get transformants... or if you get a little overzealous with your pipetting. Another is the possibility of carrying over contaminants...practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification columns...buffer bottles Fewer plastic consumables Fewer pipette tips needed in workflow Reduced waste from ...

  4. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...a single use. So how about washing and reusing pipette tips in a high-throughput and efficient way? Grenova... devices that are compatible with a variety of pipette tips. This is part of a cultural change in how ...

  5. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... Gram-negative and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different...CRISPRi to a much wider swath of bacterial species (Peters et al., 2019). The systems differ on how exactly...10: . https://doi.org/10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, ...Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA,...

  6. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ... to help them spread through bacterial genomes (Peters et al., 2017). Type I CAST systems rely on the ...CAST activity allowed certain mutated phages to outcompete their brethren, and the researchers isolated ...–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin,...

  7. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...methylation competent E. coli for this procedure to work properly. Directly transform chemically competent cells...are then transformed directly into chemically competent E. coli and transformants selected with the appropriate...

  8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse...Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just...heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively remove a stop...

  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...as a device fits on the deck, and it’s easy to pipette samples in and out of it, you can set up a procedure...using electronic multichannel and single channel pipettes. These can speed up any project in which you need... to do a lot of aliquoting. Simply program the pipette with how much total sample you need and the volume...

  10. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...that allow their host organisms to thrive in competitive environments. The genes encoding these functions...triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only organism that... are also less aggressive ways to survive in competitive environments — like by living where no one else...

  11. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad’s Pattern team,...between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally...Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, ...

  12. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...the bench and all consumables with 10% bleach.  Pipette slowly If you are using a manual droplet generator...automated droplet generator, you should avoid pipetting too quickly when making your virus dilutions. ...

  13. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...just one or two recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...(1991): 62-66. PubMed PMID: 2003926. 2. Bubeck, Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning...

  14. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...ensure that nothing is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at...Each sample that is transformed is plated onto a petri dish plate that is also barcoded. The barcodes on...

  15. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...decreasing the probability of creating replication-competent lentivirus (RCL) in your target cells.  Q4: How...targeted. Q6: Are transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer...are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential for...

  17. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...your experiments. Don’t dive directly into the pipetting! MoClo nomenclature and structure can be quite...To combat this, you can increase the volume of competent cells you plate after each transformation and/...high-throughput, we recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your...

  18. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...sample is immediately ready to transform into competent cells. The master mix of enzymes can be purchased...master mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically...terminator sequence), since this would directly compete with the required single-stranded annealing and...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...restriction-deficient; good strain for cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB...lacY1 proA2 rpsL20 xyl-5 leu mtl-1 Stbl4   Electrocompetent cells for cloning and storage of unstable ...XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids, ligated...

  20. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...
Showing: 41 - 60 of 231 results