We narrowed to 52 results for: pcas9

1. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   Published

   Feb. 14, 2018, 2:36 p.m.

   ...The PAM Requirement and Expanding CRISPR Beyond SpCas9 The closer your edit is to the PAM site, the higher...

2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

   Type

   Blog Post

   Published

   June 9, 2016, 2:30 p.m.

   ...887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307...

3. Visualizing Genomic Loci with CRISPR-Sirius

   Type

   Blog Post

   Published

   April 18, 2019, 12:53 p.m.

   ...different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to...

4. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

5. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...with these modified gRNAs is comparable to that of SpCas9. The group also confirmed that the Cas12f1 and ...

6. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...is more stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific...

8. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

9. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...also fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility,...

10. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A...

11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing...

12. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...