We narrowed to 74 results for: phage

...by using 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein ...Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their cognate.... These pairs are closely related to the lambda phage integrase system which is the basis for Gateway ...authors recently used computational protein design, phage display and high throughput binding assays, to engineer...

...Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141. PubMed PMID:...more abundant are the Earth’s estimated 1031 bacteriophages (viruses that infect bacteria), many of which...

... good at flipping. So the lab instead turned to phage serine integrases, where they saw 10-20% integration...

...Antibiotic Resistance Genes Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use...genes from one bacterium to another through bacteriophage (viruses that only infect bacterial cells), ...

...and specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate...Bauer, D. E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with... indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor ...Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors...

...Bauer, D. E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with...

...effector toxins, toxin-antitoxin type toxins, and phage holins. They hypothesized that genes that had evolved...which uses enzymes domesticated from the Lambda bacteriophage, is arguably the most widely and productively...allow replication only in hosts carrying the bacteriophage lambda pir protein. This allows conjugation ...

...hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance gal Mutation in galactose metabolism...

... homology sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice...

...systems to defend themselves against bacteriophage infection, but some phages fight back using CRISPR inhibitors...3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This toolbox...four proteins from Listeria monocytogenes (Lmo) prophage that can inhibit CRISPR function. These Lmo proteins...

...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...transcription/ general expression Promoter from T7 bacteriophage Constitutive, but requires T7 RNA polymerase... levels of gene expression Promoter from T7 bacteriophage plus lac operators Negligible basal expression...transcription/ general expression Promoter from Sp6 bacteriophage Constitutive, but requires SP6 RNA polymerase...High levels of gene expression  Promoter from bacteriophage lambda Can be temperature regulatable  Often...transcription/general expression Promoter from T3 bacteriophage Constitutive, but requires T3 RNA polymerase...

...on using bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics...engineering and scientific concepts to engineer bacteriophages and use those to detect unwanted bacteria. ...idea of starting a company around engineered bacteriophages. He decided to quit his "safety net" at the...

...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...over-express CD47 to escape recognition by circulating macrophages. Tumor cells also downregulate the expression...

...study the expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors...

...pAAV CD68-hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539  AAV5  pOTTC1596 - pAAV SYN1...

...in the scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for...

...further differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018...

...efficient gene deletion in B cells, T cells, macrophages, and dendritic cells, with no deleterious effects...

...to a self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment...