Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 72 results for: phage

Showing: 41 - 60 of 72 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... good at flipping. So the lab instead turned to phage serine integrases, where they saw 10-20% integration...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...Antibiotic Resistance Genes Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use...genes from one bacterium to another through bacteriophage (viruses that only infect bacterial cells), ...

  3. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...and specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate...Bauer, D. E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with... indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor ...Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...effector toxins, toxin-antitoxin type toxins, and phage holins. They hypothesized that genes that had evolved...which uses enzymes domesticated from the Lambda bacteriophage, is arguably the most widely and productively...allow replication only in hosts carrying the bacteriophage lambda pir protein. This allows conjugation ...

  6. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance gal Mutation in galactose metabolism...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...systems to defend themselves against bacteriophage infection, but some phages fight back using CRISPR inhibitors...3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This toolbox...four proteins from Listeria monocytogenes (Lmo) prophage that can inhibit CRISPR function. These Lmo proteins...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...transcription/ general expression Promoter from T7 bacteriophage Constitutive, but requires T7 RNA polymerase... levels of gene expression Promoter from T7 bacteriophage plus lac operators Negligible basal expression...transcription/ general expression Promoter from Sp6 bacteriophage Constitutive, but requires SP6 RNA polymerase...High levels of gene expression  Promoter from bacteriophage lambda Can be temperature regulatable  Often...transcription/general expression Promoter from T3 bacteriophage Constitutive, but requires T3 RNA polymerase...

  10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...on using bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics...engineering and scientific concepts to engineer bacteriophages and use those to detect unwanted bacteria. ...idea of starting a company around engineered bacteriophages. He decided to quit his "safety net" at the...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

  12. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...over-express CD47 to escape recognition by circulating macrophages. Tumor cells also downregulate the expression...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...to a self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment...

  20. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity purification...
Showing: 41 - 60 of 72 results