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We narrowed to 362 results for: pme

Showing: 41 - 60 of 362 results
  1. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire...computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid tools...PMC6576255. Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41...conditional protein depletion in C. elegans." Development 142.24 (2015): 4374-4384. PubMed PMID: 26552885...
  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome...health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes...timescale. Our article ultimately reported the development of a rapid design and prototyping pipeline that...necessary to advance the technology through product development, the regulatory process, and manufacturing so...
  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...18.4 (blue), 5.8 (red) 2.7/4.7 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT...subcellular location, start of gene expression, development of a cell structure, etc). FP timers should therefore... therefore find use in studies of animal development where events like the patterning of nascent tissues...fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation." Journal...
  4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...the ordered assembly of the target construct. Development of Golden Gate Assembly test systems Our research... Eric Cantor is a molecular biologist and a Development Group Leader overseeing a team bringing new products...
  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020). Benefits and... pathway to diversify science. Evolution and Development. 24(5):127-130. Kimball, C., Powers, K., Dustin... progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J....
  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...last several years, my lab has been working on development of technologies for biochemical analysis of genome...in biology. Therefore, my lab is dedicated to development of new biological technologies. The locus-specific... we've made some contributions in technology development in biology and hope to develop more in the future... of the game. In this regard, I believe that development of new technologies may be very effective in ...
  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...provide a strong proof of concept for further development. Figure 2: Mechanisms of deaminase-based...editor allows guanine transversions And further development was on its way! All base editors up to this point...Still, it opened a new avenue for base editor development going forward. Figure 3: Mechanism of ...Shi, L., Yao, X., Zhou, Y., & Yang, H. (2024). Development of deaminase-free T-to-S base editor and C-to-G...
  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...them in numerous fields including research and development, diagnostics, drug discovery, and food safety...chemiluminescent substrates are available. Labs with limited equipment often opt for the chromogenic substrates since...chemiluminescent methods that require specialized equipment. Chromogenic methods are not as sensitive, however...followed by conjugated secondary antibodies. Assay development, however, can be challenging since scientists...
  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...of our QC process. Automation and continuous development of in-house software has been crucial for us ...microplates, and thanks to our dedicated product and development teams, frequent updates allowed us to scale our...and well-supported, so we could focus on our development instead of building the entire ecosystem Proven...computing resources. Now, we could iterate our development and testing cycle faster due to the ease of jumping...
  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...and cellular biology, this happens through the development of tools that exploit and expand our current ...which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest to find,...Cas systems opens up the potential of further development of low-toxicity RNA editing tools.   Fig...embryonic stem cells and glioblastoma cells. PASTE Development and discovery The first generation of CRISPR ...
  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...University of Rochester Medical Center. During development, complex genetic programs specify and assemble...technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience...recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (...regulates neuronal physiology by promoting the development of neurons and synapses as well as long-term ...
  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...and gels). As a freshman in college I studied development and evolution of histone genes in Strongylocentrotus... in a tank, and observe the beauty of embryo development in real time. I was already a biology geek, but...that can be studied by providing support "for development of tools, approaches, and infrastructure necessary...juvenile, light microscope with polarizing filter. Development is direct in this species, and hatchlings are...
  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...embryonic development, extended-open integrins enable tissue morphogenesis, organ development, and the ...as immune response, tissue repair, and kidney development. Despite their ubiquity and significance, these...all key to basic biomedical research and drug development. Integrin structure and conformational complexity...
  14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ... were very time-intensive, necessitating the development of a new technique. In a structure as tightly...lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium. For scientists interested...interested in neuroscience and development, Brainbow is a valuable tool to mark and follow single cells... Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631...
  15. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...up to and who doesn’t care about your career development? To get a better idea of whether or not a lab...one that is a true mentor, supporting career development and growth. Have lab alumni gone into both academic...you help or at least observe grant writing? Get equipment quotes? Understand administrative responsibilities...lab help you head for a career in drug/device development? Does the advisor have industry connections such...
  16. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...limited compared to other colors. Two recent developments add to this part of the spectrum: PinkyCaMP ...experiments in classroom settings without specialized equipment (Collins & Lau et al., 2024). The method uses ...eliminates the need for expensive reagents and equipment like incubators, micropipettes, and plate readers...analyzed using either "high-tech" or "low-tech" equipment. Reproduced from Collins & Lau et al. (2024) under...
  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Repressible Binary Systems GAL4/UAS In Drosophila or development studies, you may hear some transcription factor...expression and tracing of gene expression across development. One common binary system is the GAL4/UAS system...place the GAL4 gene downstream of a tissue- or developmental stage-specific promoter and design a UAS reporter...PMCID: PMC3811519.Seo SO, Schmidt-Dannert C. Development of a synthetic cumate-inducible gene expression...
  18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...et al., 2006). Despite its relatively recent development, the history of optogenetics begins almost 50...where she worked as a post-doctoral fellow on developmental biology at the École Normale Supérieure de Lyon...Lief, Ofer Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review...Krook-Magnuson. "Specificity, versatility, and continual development: the power of optogenetics for epilepsy research...
  19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...mobility are essential processes during embryonic development. These are required for a growing organism to... follow immune cells in lymphoid organs. The development of cell sorters together with the engineering...cell.     The past 15 years have seen a lot of development surrounding the use of fluorescent proteins and.... "Macrophage and T cell dynamics during the development and disintegration of mycobacterial granulomas...
  20. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...find and test genes that may play a role in the development of uterine tumors. According to Dr. Bengyella...will be open to researchers who have received equipment through Seeding Labs’ Instrumental Access program...future. Additional Resources Apply to Recieve Equipment through Seeding Labs' Instrumental Access Program...
Showing: 41 - 60 of 362 results