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We narrowed to 36 results for: pme

Showing: 1 - 20 of 36 results
  1. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...protective equipment (PPE) for BSL-1 and BSL-2 labs.... Protocols Personal Protective Equipment (PPE) for ...for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Intro to the Lab Bench...Watch the video for tips on using PPE in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection...
  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...When handling, use proper personal protective equipment including laboratory coat, laboratory goggles,...Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety Cabinet 4 ...498 Before Starting Wipe down all pipettes and equipment with 10% bleach prior to use. Warm the affinity...
  3. Centrifugation

    Type
    Protocol
    ...2022 Equipment Centrifuge Microfuge tubes or other suitable container Personal protective equipment (PPE... A centrifuge is a common piece of laboratory equipment used to separate different components in a liquid...centrifuge before you and may still be on the equipment. Before using the centrifuge for your samples,.... Improper use of a centrifuge can damage the equipment, your samples, or you, so be sure to keep your...
  4. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...cells Day 3-6: Feed cells Day 7: Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes...Preparation instructions. Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent Preparation...
  5. Immunocytochemistry

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...Day 1: Seed cells Day 3-4: Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking...
  6. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...recombinant antibodies using Coomassie stain. Equipment Heat block 1–10 µL single channel pipette 2–20...
  7. Western Blot

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...visualize a protein from cells or tissue samples. Equipment Microcentrifuge 0.5–10 µL single channel pipette...
  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and reagents...not endorse or recommend specific products or equipment. Inclusion of this information is solely for transparency...available, use an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates...
  9. Kit Free RNA Extraction

    Type
    Protocol
    ...quickly to reduce RNA degradation. Keep work area, equipment, and reagents RNase-free (Use an RNase decontamination...extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated microcentrifuge If you only have ... you’re done using the centrifuge, bring this equipment back to room temperature, as prolonged storage...
  10. Weighing Reagents Protocol

    Type
    Protocol
    ...appropriate personal protective equipment (PPE) prior to handling the materials. Equipment Weighing boats or weighing...
  11. Water Bath Protocol

    Type
    Protocol
    ...Introduction A water bath is a piece of laboratory equipment that helps bring your materials to a particular...video for tips on using a water bath in the lab. Equipment Water Bath 10% bleach or 70% ethanol Distilled...
  12. Video Library

    Type
    Protocol
    ...aseptic technique, including personal protective equipment, setting up a clean workspace, and maintaining... Joel McDade Joel McDade, Addgene's Business Development Manager, discusses his transition from the Scientist...
  13. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... video to learn more about safety in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection...the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you are working...
  14. AAV ddPCR Titration

    Type
    Protocol
    ...with your institution’s biosafety regulations. Equipment Class II, Type A2 Biological Safety Cabinet, Labconco...values, we recommend wiping down all pipettes and equipment with 10% bleach prior to use and keeping all reagents...
  15. CRISPR Library Amplification

    Type
    Protocol
    ...ensure that outgrowth and competition are limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser...and follow all specifications described in the equipment manual. Immediately add 1 mL SOC to cuvette. Remove...
  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ... and harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety Cabinet Aspirating...Troubleshooting We recommend wiping down all pipettes and equipment with 10% bleach prior to use and keeping all reagents...
Showing: 1 - 20 of 36 results