We narrowed to 100 results for: sag

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ...CUT&RUN protocol, the most significant being the usage of a strong detergent to permeabilize cells rather...

2. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...s level of discomfort appropriately to get the message across. Listen to our podcast interview with Harvard...

3. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...perfect plasmid backbone. Or just ask –  leave a message in the comments below and an Addgenie will help...

4. What the HEK?

   Type

   Blog Post

   Published

   April 18, 2023, 1:15 p.m.

   ...to maintain short culture periods (less than 20 passages) to prevent these issues from popping up, and ...

5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   Published

   March 5, 2020, 2:15 p.m.

   ...data. These data focus on experimental design and usage of these tools, which aren’t always easy to find...

6. Safe Port in a Storm...How Addgene is Weathering the Pandemic

   Type

   Blog Post

   Published

   Sept. 17, 2020, 1:15 p.m.

   ...they were very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after...

7. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

   Type

   Blog Post

   Published

   Aug. 8, 2019, 1:19 p.m.

   ... of the paper, I usually try to grasp the main message of the work. I underscore the sentences that I ...

8. RUBY-Red Siliques

   Type

   Blog Post

   Published

   March 1, 2022, 2:15 p.m.

   ...selection of transformed plants to decrease the routine usage of antibiotics which may contribute to the emergence...

9. Viral Vectors 101: Transductions

   Type

   Blog Post

   Published

   April 30, 2024, 1:15 p.m.

   ...considerations, they aren't dangerous for controlled lab usage. Using viral transductions can offer benefits such...

10. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...derived from the same cell type; however, their usage has been limited by technical problems. For instance...

11. Mentoring for Scientists: I Have a Mentor, What Now?

    Type

    Blog Post

    Published

    March 6, 2014, 7:12 p.m.

    ...gym every day. If I skip, I get a bunch of text messages asking me, “What happened? Where are you?” My ...

12. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    Published

    April 4, 2023, 1 p.m.

    ...powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted for the ...

13. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    ...our pets. #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing...

14. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type

    Blog Post

    Published

    June 30, 2020, 1:15 p.m.

    ... with the author. “In the app, you can write a message to the author of the poster,” he says.Viewing a...

15. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ... variants in vitro against different drugs and dosages.  Michal Bernach: Developing an artificial leaf...

16. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...

17. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    Published

    Feb. 9, 2021, 2:15 p.m.

    ...shift working and social distancing. The take home message is the approaches/tools we have to foster creative...

18. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    Published

    Jan. 12, 2021, 2:15 p.m.

    ...I accepted the connection invitation. They now message me frequently and ask me how I’m doing with nothing...

19. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...simultaneously. Do note for control purposes that there are dosage effects. For example, overexpression of two genes...

20. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...experiments and offer flexibility in tag, cleavage, codon usage, and expression system. Mammalian expression plasmids...