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TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...issues of diversity in science. A great person to contact is Twitter’s @DrQualls. Marquita Qualls of Entropia...content is of great value. I have never been in contact with Aida Baida Gil of Coach De La Professional...
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Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...resistance plasmids, which protect the host from attacks by other organisms. To conserve energy, these specialized...genetic material to a recipient through direct contact. That’s conjugation! F factors can also be integrated... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....which means you’re more likely to have the right contacts and get your resume pulled to the top of the pile...position. Work your LinkedIn connections and other contacts to get that referral and find out more about the... postdoctoral experience Read Joanne Kamen's 8 tactics for choosing a lab Resources on Addgene.org Find... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....genome editing applications. So, how does NgAgo stack up thus far? A few weeks ago, I was going through...suggestions and tips in the comments section below or contact [email protected] if you'd like to write a more ...Specificity with eSpCas9 and SpCas9-HF1 See How Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org ... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....educating the public and organizing events. I contacted local professional organizations and nonprofits...Science Indiana and sent emails to any group with a “Contact Us” button on their website. Then I had a lot of...Without the encouragement and pep talks from these contacts, I would have quit my “nontraditional” career ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....after all, among the more virulent viruses (it attacks the immune system directly). It may seem odder ...6 x 106 virus-like particles that we come into contact with every day (Prussin et al., 2015), only a small...what these vector particles do once they come in contact with target cells (Fig. 3). Figure ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....all better” for you, I can offer some tips and tactics that have worked for my friends, my colleagues ...at the same time. Use time management tools and tactics to plan your work and make this happen. Be realistic...professional community that you can count on for tips and tactics that will help you do your job better. #8 Thou ... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....needed to find the right contacts at the companies and then convince the contacts to give me some time to...collaborators’ professional developments without directly contacting them. One great way to transition from an internal... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m.... an appropriate job opening, you can send your contact an email with the relevant info and they just might...receive such a call, make sure to get that person’s contact information and again, make a personal connection...information, but not a job! This is good etiquette when contacting someone professionally that you do not know very... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....antibiotics, like penicillin. These specific drugs attack the cell wall of bacterial cells; however, Mycoplasma..., is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so that exposure... public health. References 1. O'Neill, J. "Tackling drug-resistant infections globally: final report... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....you to visualize a target of interest within an intact cell, tissue, or, in some cases, a whole organism...use of antibodies to visualize a target within an intact sample, a cell in the case of ICC or a tissue in...antibody targets the antigen of interest but is not attached to a fluorescent molecule, instead a secondary... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...allowing the endogenous signaling pathway to remain intact and be studied without confounding issues associated... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....piggyBac™, the cargo is inserted into the genome fully intact, while, with a standard DNA plasmid transfection...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....wall. Here, I will cover some of the best ways to tackle mycoplasma contamination before they enter your...dry state for a longer time. When they come in contact with a nutrient source, they start proliferating...while working and discard them after use. Human contact can spread contamination when you are working without... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....community of creative scientists delivered some spectacular photos once again this year! All winners receive...and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis) ... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog PostPublishedFeb. 27, 2020, 2:15 p.m....updates regarding the order will be sent to the contact email addresses provided. How do I place an order...billing address for your organization. We will contact you directly if we require a copy of your PO. ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....single consensus lentiviral expression vector and attached a DNA barcode to each to make the library usable...certain environments, for example. “We’ve been contacted by investigators all over the world who are interested... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m.... inspired to tackle your next science challenge. If you have any feedback, please contact us and let us...