...very specifically to target molecules. Using a technique called systematic evolution of ligands by exponential... used in a variety of applications, including detection of environmental pollutants, drug discovery, and...a “bad batch”); Customizable sensitivity and detection - since they frequently undergo conformational...oligonucleotides in fluorescent visualization techniques for decades, most notably for fluorescent in ...visualize RNA. Low signal-to-noise ratios with such techniques can be problematic, however. Well designed aptamers..., thereby improving traditional visualization techniques. In addition, their incredible versatility opens...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...

...disease earlier. New techniques are currently being tested in clinical trials to detect mesothelioma earlier...diagnosis, the hope is that as these new detection techniques and therapies emerge into mainstream use... asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe level of asbestos...growth of tumors. More often than not, multiple techniques are used as part of a treatment plan, an approach...intraperitoneal chemotherapy (HIPEC). With this technique, surgery is performed to remove the mesothelioma...delivered intravenously. New treatment options: Early detection, immunotherapy, and angiogenesis inhibitors New...life expectancy of mesothelioma patients: early detection and immunotherapy.  It has long been known that...

...people find it can detect even smaller differences, and it can even be used to detect a single copy of ...which aims to increase your understanding and technical proficiency with common applications - now let... thumb, you can assume that qPCR can accurately detect expression differences of twofold or greater with...Can be multiplexed Single amplicon validation technique Hydrolysis Probes (Taqman) Low; can be reduced...Biassoni, 2014). At the bench As a highly sensitive technique, qPCR needs a steady pair of hands to produce ...of triplicates to understand variation due to technical error.  Plate readouts can be affected by plate...differences may be a concern in your reaction, analysis techniques that use raw data corrections are a better choice...

...contaminants and to subsequently develop technologies to protect fresh water repositories in Mexico. At ...insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides as well...a bacterial expression system for renewable biotechnology products Dr. Idah Sithole-Niang, University ... Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to preserve Cassava yields ...Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy DNA...Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally and...Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research involves...

... a revolution in genome engineering, with new techniques rapidly being developed. Performing a CRISPR ...potential for off-target effects. Cas9 RNPs are detectable at high levels shortly after transfection, and...plasmid based methods. One of the most common techniques for delivery of RNPs is electroporation (A in...the cytoplasm. In addition to the use of this technique in cell culture, it has also been applied to genome...combined with cell-type specific reagents in a technique known as nucleofection, which forms pores in the..., allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection (B in the...using this method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands...

... commercially available mycoplasma detection kits that use detector cell lines, ELISA, fluorometry, or...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed... lines, or infected personnel and can persist undetected; unlike infections with larger microbes such ...or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the...or PCR-based approaches to detect even low levels of contamination. For those labs that prefer a more ...more economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with...alters microarray gene expression profiles.” Biotechniques 35.4 (2003). 812-4. Pubmed PMID: 14579747. 3...

...Sample preparation for these techniques sometimes makes it more difficult to detect protein tags using antibodies...availability of reliable primary antibodies for their detection, particularly when antibodies to the POI are non-specific...multimeric tags are frequently insufficient for detection when the POI is weakly expressed. As these epitopes...have no intrinsic visual signal, they cannot be detected directly in live imaging experiments. It is therefore... are both intrinsically fluorescent and can be detected with highly epitope-specific, fluorescently-labeled...can be easily expressed in the brain and can be detected through their direct fluorescent signals or using...antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for weakly...

...consists of prominent members of the bioscience and tech transfer communities who help guide and promote ...that had Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success ...biological questions, you need a tremendous amount of technology development alongside it.” Doench starting working...Perturbation Platform and encompasses a variety of technologies to functionally investigate the human genome...genome. Doench says he enjoys working on technology development under the Platform model at the Broad Institute...Institute because they are “not just developing technology for its own sake,” but work in true collaboration...history of RNAi left him a bit skeptical of the technology at the start of the CRISPR craze, but he says...

...microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques is Best...fluorochrome is removed from all other detectors except its dedicated detector. To ensure proper compensation ...cell health, analyzing cell cycle stages, and detecting specific populations of cells in a complex tissue...sample (1). When combined with cell sorters, this technology can be used to isolate a specific subset of cells...fluorescence intensity.   What flow cytometers detect Cell size and complexity, measured using visible...directions. The degree of scatter is captured by detectors and provides useful information about the cell...emits a fluorescent signal that is captured by detectors. The strength of the emitted photons varies from...

...CRISPR 101" post. Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive... assays (e.g. Surveyor® or T7E1) for mutation detection. While these methods paved the way for mutation...introduce digital PCR as an emerging validation technology. Digital PCR has several advantages over mismatch... require standard curves, and can theoretically detect a single target molecule in a sample. Digital ... real time PCR. These assays can be designed to detect either a donor sequence integrated through homology-directed... remainder of this post will focus on assays to detect NHEJ. These assays include a forward and reverse...between 200 and 500 ng of purified PCR product. Low detection limits: Digital PCR assays can accurately quantify...

...popular technique in neuroscience and developmental biology. This type of cell labeling technique involves...group developed the original iteration of this technology in 2007. Used to label neurons in the cerebellum...cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores ...construct but do not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1...CMV-Brainbow construct at the embryonic stage. This technique could be used to target Brainbow to neurons by... promoter. Zebrabow is an extremely versatile technology as recombination can be induced in developing...cells. Tetbow optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the...

...Einstein College of Medicine has recently published techniques to follow translation in living single cells....cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even here, there is the question... symposium seeking inspiration to resolve the technical hurtle of monitoring protein turnover of specific...interesting talk by Prof. Erin M Schuman, a former Caltech researcher, now Director at the Max Planck Institute...Frankfurt am Main, Germany. She presented a novel technology, back then still unpublished, that allowed the...synthesized proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical...noncanonical amino acid, azidohomoalanine (AHA). In the technique, AHA is incorporated into newly synthesized proteins...

...Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer...is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot on what...Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA...that can greatly expand gRNA options. Two newer technologies offer an alternative to HDR for introducing ... another target C or A in the window. Another technology, prime editing (reviewed in Anzalone et al., ... although these are still early days for this technology, and the user may need to optimize numerous parameters...CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near...

...Importantly, conventional flow cytometry uses one detector to detect one fluorophore and therefore one cellular...fluorophores can “spill over” into detectors that are not the primary detector for that fluorophore. For example...single (primary) detector to identify a fluorophore, spectral flow cytometers use all detectors for all fluorophores...panels: five laser spectral flow machines have 64 detectors, allowing for the use of up to 64 markers in a...emitted by a fluorophore is guided to a single detector using a series of mirrors and filters. Importantly... FITC signal is spilling over into the primary detector for PE. In order to correct for this, compensation...the fluorescent molecules can be quite broad, detectors will usually only pick up a small portion of that...

... and can be used in a multitude of scientific techniques such as molecular cloning or molecular diagnostics...used for an array of different molecular biology techniques and experiments. End point PCR’s goal is to amplify... cloning to create a plasmid of interest, for detecting the presence of an insert or piece of DNA in a...reaction (qPCR) also known as real time PCR is a PCR technique used for measuring a starting DNA concentration...during the PCR reaction cycles is continuously detected via a fluorescent signal. The signal increases...concentration. The cycle in which the unknown DNA is detected compared to the standard curve can be used to ...right. ddPCR uses a water-oil emulsion droplet technology that fractions the PCR reaction sample into approximately...

.... However, most SRM techniques also require specialized instruments and technical expertise, limiting ... model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...biological features previously only visible via techniques like super-resolution microscopy or electron ...Super-resolution microscopy (SRM), a family of imaging techniques developed to break through the resolution barrier...some of the limitations?  A “super-resolution” technique that uses common reagents, straightforward protocols...there is always a catch - some limitation of the technique that users should be aware of - and ExM is no ...microtubules. This issue is shared by other SRM techniques and can be alleviated by maximizing label density...

...learning a new technique while visiting a lab, or maybe even training on a new technique with a coworker...research group, you will also typically receive technical mentorship from a ‘bench mentor’. In some instances...mentor is a staff scientist, postdoc, lab manager, technician, or a graduate student. This is the person you...basis who will help you get on your feet with techniques in the lab (and possibly much more, depending...difficult for you to become proficient in lab techniques, which will hinder your progress. You will also...serve two purposes – familiarize you with the techniques you will be learning and the biology you will...there for you to be independent performing the technique you trained to do? Walk through the protocol mentally...

...Förster) Resonance Energy Transfer, technique which allows the detection of molecules' interactions, modifications...fluorophores to proteins, pioneers of this technique were able to directly detect protein/protein interactions in...dissociations in situ. Used since the mid-90s, this technique has revolutionised the way we apprehend molecular...playing “live.” Indeed, FRET was one of the first techniques which enabled the measurement of single molecule...microscope. Historically, molecular interactions were detected by indirect means often using probes with the ... fluorophore can be measured using microscopy techniques. FRET measurement sensitivity makes it suitable...DNA and proteins. FRET, in theory, is an easy technique, but it is very important to follow some simple...

...chromosome, which was technically challenging. Other homology based technologies We've described Sequence...alternatives to restriction enzyme cloning. These newer technologies have become more and more common, and for good...Overview of Gibson assembly The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues... 5 days. One drawback to the Gibson assembly technique is that the process works best with fragments ...Gibson assembly combined with the popular CRISPR technology and is described in a recent publication by the...When followed by the standard Gibson assembly techniques described above, this resulted in the direct ...Cloning (System Biosciences) In-fusion Cloning (Clontech) Although these kits may come at a high price...

...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over... Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant...but it has precluded widespread adoption of the technology. In 2012, Saito et al. described the first Nano-lantern...luciferase (RLuc8) with Venus. This luminescent technology is based on the principle of bioluminescence ...to excite the fused fluorescent protein. This technique is very similar to the widely used FRET, but it...ATP sensors based on Nano-lantern, showing the technique’s versatility and adaptability to many different...optogenetic system. Biosensors based on Nano-lantern technology are also very robust, with an eNL-based calcium...