...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...indel generation for two PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing...3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly higher mutation...mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How many loci can...gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted genomic sites separated...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques...

...stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose, immune, inflammatory...cells are Cancer-Associated Fibroblasts, or CAFs. CAFs are altered fibroblasts and promote the development...gland biology and neoplasia 10.3 (2005): 249-260. PubMed PMID: 16807804. Hanahan, Douglas, and Lisa M. Coussens...Addgene.org Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about...began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered in microenvironments...Saidou, and Kounosuke Watabe. "Cancer associated fibroblasts (CAFs) in tumor microenvironment." Frontiers ...

...both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...publicly available 2D/3D mouse atlas from antibodies that we screen. This atlas will enable scientists to ...and uploading verified antibodies onto the mAb3D atlas website. This website can then be used by thousands...distinct imaged pattern should align with what the atlases (mentioned above) and literature suggest, providing...distinct imaged pattern does NOT align with the atlases/literature and is unrelated to the target protein...of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation...closer to resolving the antibody crisis. The mAb3D Atlas is a public service to the science community, and...

...Addgene.org Find plasmids from the Bethune Lab Find Plasmids from the Roux Lab Find Plasmids for FRET ...BirA* fusion proteins are expressed in cells via plasmids or by the creation of stable cell lines and these...interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID and BioID identify proteins that interact...Interested in using Split-BioID with your research? Plasmids are available from Addgene! References Schopp... Central PMCID: 5467174. Find the Split-BioID Plasmids Here! Munter, S.D., Görnemann, J., Derua, R.,...site than in Schopp et al. Find these Split-BioID Plasmids Here!   Burke, B., Kim, D.I., Roux, K.J., & Raida...PubMed Central PMCID: 3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet...

...Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3 is part of the Class 1 CRISPR family...complexity. Figure 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector...out the brunt of the mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-...CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different types, with...making up type I, the most abundant type in the Class 1 category. These type Is can then be further broken... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...Koonin, E. V. (2015). An updated evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology...

...packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called...blog Parts of the AAV Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables That Matter AAV protocols...called “triple plasmid transfection” (Figure 1).    Figure 1: Overview of key steps of AAV production, iodixanol...repeats (ITR). You should always have a high-quality plasmid standard and rAAV reference material of a known...before undergoing titering, to remove residual plasmid DNA carried over from the production process. For...

...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds...efficiently subcloned into the phagemid pDX_init plasmid, using the fragment exchange (FX) cloning technique... subcloned into pBXNPH3 or pBXNPHM3 expression plasmids for the production of tag-free proteins for further...

...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB... tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred to recipient...transposon containing the CRISPRi components and a plasmid with the Tn7 transposition genes are transferred...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...

...lentiCas9-blast. A549 cells were transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days...18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells were then used ...expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...viruses, but you can find a list of validated gRNA plasmids on our website. There are a few reasons to target...

...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or inoculation...that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack chairs, and synthetic...other scientists save resources by sharing plasmids Find plasmids at Addgene ... popular theme in life sciences, some of these plastics are high grade and can be washed and reused. This...tips cost in half, not to mention the amount of plastic you’ve saved. Repurposing regulated medical waste...use this material to produce various grades of plastic lumber and distribute it to industrial and residential...