We narrowed to 855 results for: NTS

...it's time to take a look at some of its parts. Elements such as the antibiotic resistance gene and the...polymerase binding site) and operators (response elements). Promoters are about 100 to 1000 base pairs long...RNAP can recognize and bind to specific promoter elements. This means that the promoter present in your ...various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters and there is very... other regulatory factors: enhancers, boundary elements, insulators, and silencers; however, some "leakiness...typically include some combination of other promoter elements, and tend to be more tightly regulated. Common...ubiquitin gene Constitutive  Gives high expression in plants. H1 small RNA expression shRNA From the human...

...genome editing in plants While there have been many Cas nucleases developed for plants, it has been difficult...Encyclopedia of DNA Elements (ENCODE), a consortium aimed to identify functional elements in the human genome...specificity of multiple wild-type and engineered variants of Cas nucleases. In doing so, they also created... toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find the plant genome...

...and Harvard. Students also partner with independent career organizations to get the grants and programs...recently appeared in eLife: "A Fair Deal for PhD Students and Postdocs" by Dr. Henry Bourne, Professor Emeritus...proactive role in their non-bench training. Grad students at Washington University founded a successful ... enough. There are still university research departments that have no formal career resources for science...science trainees at all. For grad students and postdocs interested in taking matters into their own hands...

... single enzyme to cut fragments that are very different.” Because the fragments produced will have different... in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University...GreenGate includes the six most frequently used elements in plant expression cassettes: plant promoters...resistance cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available ...required to generate multi-construct transgenic plants. In the most practical terms, what GreenGate can...

...TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration...RvLEAMshort to the sample before plunge freezing represents a convenient, effective approach to protecting...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...latest work evolves and engineers Bxb1 recombinase variants to optimize PASSIGE efficiency (Pandey et al.,...allowing the team to develop self-silencing CHARM variants that stop their own expression after silencing...

...our understanding of biological processes and developments. Model organisms often feature genetic, physiological...candidate for introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia...stroke and hypertension (Iannaccone et al., 2009). Rodents have also been routinely used in an array of neuroscience...expected to shed light on human aging pathological developments (Vieira et al., 2019) Besides their ability ...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...Addgene’s plasmid collections to help start your experiments! References: Blount, Z. D. (2015). The unexhausted... A., Wells, K. M., & McCusker, C. D. (2019). Advancements to the Axolotl Model for Regeneration and Aging...

...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...inflammatory therapeutic applications. Antibody fragments such as scFvs are also used for eye diseases, ... Function, and Clinical Prospects of Antibody Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028...Applications of Single Chain Fv (Variable Domain) (scFv) Fragments. Antibodies 2:193–208 . https://doi.org/10.3390... Honegger A, Plückthun A (1998) Antibody scFv fragments without disulfide bonds, made by molecular evolution...Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390/ijms21176324...

... and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body...crucial for their function. With the right research reagents, researchers can measure this conformational change...lab, and IPI — used IPI antibodies and affinity reagents to study the difference in cell morphology changes... teams employed IPI synthetic antigen-binding fragments (Fabs) that bound an amino acid region displayed...containing the αV subunit. Using these affinity reagents, the investigators inhibited other RGD-binding...collaboration and education in protein science, IPI hosts events and training courses on technologies and laboratory... on the IPI website and stay informed on future events by signing up for the IPI newsletter. The collection...

...to the start of another academic year, graduate students and post-docs alike are wondering where the time...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback. ...goals and effort needed to complete them. These documents dictate the subject matter discussed at the meeting...have accomplished. Starting with a recap of accomplishments, from learning new techniques to finally figuring...development - let us know your strategies in the comments section!   References 1. Yearly Planning Meetings...

...are many combinations to choose from for your experiments! Late last year, we hosted our first AAV Education... main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector into your... nontransgenic animals by using cis regulatory elements such as promoters and enhancers, multicistronic...multicistronic vectors, and post-translational regulatory elements. Examples of these approaches are given in Tables...development of this practical guide by the workshop participants  - a testament to openness and sharing in science...the neuroscience community by sharing not only reagents, but also method information. To complement these...