We narrowed to 813 results for: TIM

...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

... a catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use lentiviral...

...interaction is typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

...Feinberg and Bert Vogelstein showed, for the first time, there were unusual patterns of DNA methylation ...undetectable amounts: ergo, DNA demethylation! With optimization, this method can produce nearly 100% demethylation...

...modifying these tools for COVID-19 detection with the ultimate goal to develop low cost and quick point-of-care...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about it ...

...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

...modifications in real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge...

...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

...without the need to find a llama farm! To find an optimally binding sybody, you want to start out with a large...protocol goes through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the...

...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

...eclosion at the start of the day and throughout daytime. They mostly stop emerging at night. An incubator...recombine chromosomes during meiosis. This can sometimes be a handy feature when setting up genetic crosses...

...interest. Scientists are continuously updating and optimizing FP tools to avoid these problems. See, for example...example, the recently developed FPs optimized for diverse cellular environments. Since colocalization ...

...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...