We narrowed to 841 results for: Abo

... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

...targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing.  For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below...

...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...

... extremely heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation...favor accumulation of the latter products. As noted above, a single cycle of cleavage and accurate repair ...

...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...writing! Let us know if you have any other questions about writing and publishing your manuscript, or add your...

...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...were observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9...

...Fluorescent 101 blog posts Read about chemogenetics vs. optogenetics Find more about the many types of protein...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...talking about Click Chemistry since 2001, making use of robust reactions that work in just about any situation...

...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...available systems. Producing lentivirus: it's all about the T's (transfection and titers) To produce lentivirus... optimize transfection conditions. As mentioned above, plasmid ratios can be adjusted to enhance titer...

...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology... Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids ...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...protein activity while also capturing information about spatial and temporal organization. The design use...