We narrowed to 872 results for: FER

...elements. Regulatory DNA function is typically inferred based on correlative biochemical characteristics...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...Aug 1;289(31):21312-21324. PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information ...

...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX...human cells. Using the structure of CasX at eight different states of assembly from cryo-electron microscopy... Jennifer Tsang...

... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

...per experiment. In the McKay lab we study how differential access to transcriptional regulatory elements...nuclei extraction. They report that this change offers a significant improvement and simplification of...cell or tissue preparation.   All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in ...

...room for serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments...project's potential. Widening your search creates fertile ground for serendipitous encounters that could ...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...

...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ...

.... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ...

...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... single-stream or city recycling services don’t offer plastic recycling, specialized companies can recycle...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender...

...were really leader-specific. How is leadership different from good management? Aren't all career skills...because it emphasizes the importance of these very different roles. At any stage in our careers we may be in...boss) that you are ready to try operating in a different role?  I think the secret sauce can be summed ...

...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

...want to offer a huge thanks to all the Addgenies who worked so hard to make this poster, offered suggestions...of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene...

...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

...genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and ease of use....moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

...enable the conversion of RNA templates to DNA but suffer from several important shortcomings including low... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... Jennifer Tsang...

...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

...)    Science communication, or science writing, refers to the process of communicating science to laypeople...knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers ...field-specific terminology or language. This can refer both to terms that are only used in the field and...

...will help with the next steps.   References and resources References Al-Allaf FA, Tolmachov OE, Zambetti...Choose a restriction enzyme that will give you different band patterns for the recombined vector backbone...

... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a... Jennifer Tsang...