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Showing: 581 - 600 of 895 results

  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than... monitored throughout production, ensuring the identity of each sample from the DNA tube all the way to... preparation. After production, we confirm the identity of the transfer plasmid in the viral preparation...

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...negative staining. Empty vector particles can be identified after negative staining and appear darker than...been used to titrate AAV, but this method is not widely used today (Fagone et al., 2012). PCR-based methods...from different sources in the same experiment, consider retitering both AAV in your lab. The absolute ...does not give an indication of infectious titer, consider titrating each lot of AAV in vivo to determine...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

  3. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... one bacterium sharing genes with another, an accidental transmission of genes from one bacterium to another...tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance on...different reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment... antibiotics and which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type of RNA transcript...transcript     Description Expression Additional considerations CMV General expression mRNA Strong mammalian... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ... used for Description Expression Additional considerations T7 in vitro transcription/ general expression...therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters as described...

  5. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics Guide  ... our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful resource...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary to their target sequence...plasmid and call it a day. You need to include both sides of the complementary RNA duplex and the hairpin ...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...time you need to knock down a gene, maybe you’ll consider using RNAi! References and Resources References... R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), ...

  7. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...so we’ve been busy updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers...week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not into Twitter, we have some exciting...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...other methods below should help in multimer identification.   Fig. 2: Gel image of Plasmid 61564...used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services using long-read...within a plasmid and, therefore, allow for the identification of plasmid multimers. Capillary Gel Electrophoresis...laser-induced fluorescence (CGE-LIF) for detection and can provide data on the quantity of various topologies and ...

  9. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...this concept in his microbiology lab course, he decided to add a bit of fun. He mixed together 10 E. coli...host strains affect transformation efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course...plasmids here, and check out our streak plate protocol video on Youtube:     Additional resources on the...plasmids at Addgene Check out additional written and video protocols ...

  10. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...insights into the process of getting an MD PhD, the inside scoop on developing new CRISPR tools, and the potential... : Alex introduces himself and explains why he decided to do an MD PhD - it's all in the family. 3:56 ...Biologically Inspired Engineering and why Alex decided to work there. 13:22 - 22:07 : Alex's projects ... Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn ...

  11. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ... In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...compatible with the introduction of unique molecular identifiers, which can improve the statistical power of in... of the pRDA_526 vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses...Context-Dependent Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System...

  12. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...some good guidelines to follow in order to ensure that people can quickly and easily identify what your...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

  13. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...citations for older publications. (As an interesting aside, Nature recently reported that the most cited work...their own libraries and offering another way to consider and quantify the impact of scientific publications...find ways to capture scholarly work that exists outside of traditional scientific journals. Of course, ...

  14. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...communication? It is important to identify your audience and consider their background. Social media is...contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your top tips ...communication posts Sign up to receive our science career guide Learn about different careers in science communication...

  15. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...puzzle fell into place, as they, taken alongside the insights provided by the studies in Tsien lab, covered...victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of A. victoria there is a ring...1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein could be used...

  17. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit...fixable dye stains that react with free amines on and inside the cells and are thus typically used for distinguishing...vector.) In our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction...should exclude APC-Cy7 from the rest of our panel. Besides some background cell death constantly happening...Compensation Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibody, which recognizes the primary antibody and provides the method of detection. You’ll also want to use...is used to detect proteins on the surface of or inside whole cells. Antibodies - typically, primary antibodies...staining. This panel of antibodies is used to both identify the cell subsets of interest from among the mixture...This could be cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture...IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978...

  19. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    .... Although my interests are very clear, I had no idea how to translate them into real world careers. I...this new virtual world, I was worried about how my ideas would be translated through Zoom calls and Google...frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for what my Instagram...with Quintin Marcelino who is Addgene’s Science Video Production Specialist and Arthur Guerra who is a...

  20. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...Cre - A useful tool for neuroscientists AAVs are widely used by neuroscientists because they efficiently...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. For example...only be expressed in neurons. Indeed, this AAV provides you with a means to specifically activate expression... viral vector blog posts for many more tips and ideas on how to use these fantastic tools in your experiments...
Showing: 581 - 600 of 895 results