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Showing: 581 - 600 of 902 results

  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than... monitored throughout production, ensuring the identity of each sample from the DNA tube all the way to... preparation. After production, we confirm the identity of the transfer plasmid in the viral preparation...

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...negative staining. Empty vector particles can be identified after negative staining and appear darker than...been used to titrate AAV, but this method is not widely used today (Fagone et al., 2012). PCR-based methods...from different sources in the same experiment, consider retitering both AAV in your lab. The absolute ...does not give an indication of infectious titer, consider titrating each lot of AAV in vivo to determine...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...ternary complex with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2...important functional properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024...CRBNmidi as a promising tool for future structure-guided drug design and studies of protein ubiquitination...showed greater photostability and also matured considerably faster, with expression levels comparable to...expression and removing the sgRNA plasmid after identifying successful knockouts limits further cutting and...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...Mortensen et al., 2019). Our method might provide some useful guidelines for the development of transient transformation...transformation systems for other species as well. Things to consider in developing a transient leaf transformation ... promoter in Agrobacterium. This can be easily avoided by the use of introns, which will be removed from... to quickly assemble vectors and test different ideas. We are a huge fan of the Golden Gate based MoClo...

  5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...that evolved in the last 10,000 years (which is considered recent). They have diverse traits with good genetic...control who a female wants to mate with and if she decides to raise their offspring or leave them to their...a cool fluorescent indicator system to rapidly identify recombinants. Arthropods (Jason Rasgon) This lab...

  6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...Hedgehog but this is not a direct reference to the video game character but rather to an extremely important...Christine Nusslein-Volhard and Eric Wiechaus first identified the hedgehog gene in Drosophila. They observed...nonsyndromic holoprosencephaly where the brain fails to divide properly into the right and left hemisphere. Addgene...origins of the name but some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers...Additional resources on the Addgene blog Read our Quick Guide to Working with Drosophila Read our Plasmids 101...

  7. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...research papers that I needed to read. Once I had an idea of the themes in the field, I searched for recent...field. I made sure to find information from genome-wide studies, as well as results from smaller and more... or popular papers, but looked for papers from a wide range of authors. Just start writing When I first...Plasmids Review the CRISPR Field with Our CRISPR Guide Pages ...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... one bacterium sharing genes with another, an accidental transmission of genes from one bacterium to another...tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance on...different reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment... antibiotics and which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type of RNA transcript...transcript     Description Expression Additional considerations CMV General expression mRNA Strong mammalian... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ... used for Description Expression Additional considerations T7 in vitro transcription/ general expression...therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters as described...

  10. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics Guide  ... our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful resource...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary to their target sequence...plasmid and call it a day. You need to include both sides of the complementary RNA duplex and the hairpin ...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...time you need to knock down a gene, maybe you’ll consider using RNAi! References and Resources References... R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), ...

  12. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...so we’ve been busy updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers...week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not into Twitter, we have some exciting...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...other methods below should help in multimer identification.   Fig. 2: Gel image of Plasmid 61564...used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services using long-read...within a plasmid and, therefore, allow for the identification of plasmid multimers. Capillary Gel Electrophoresis...laser-induced fluorescence (CGE-LIF) for detection and can provide data on the quantity of various topologies and ...

  14. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...insights into the process of getting an MD PhD, the inside scoop on developing new CRISPR tools, and the potential... : Alex introduces himself and explains why he decided to do an MD PhD - it's all in the family. 3:56 ...Biologically Inspired Engineering and why Alex decided to work there. 13:22 - 22:07 : Alex's projects ... Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn ...

  15. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...this concept in his microbiology lab course, he decided to add a bit of fun. He mixed together 10 E. coli...host strains affect transformation efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course...plasmids here, and check out our streak plate protocol video on Youtube:     Additional resources on the...plasmids at Addgene Check out additional written and video protocols ...

  16. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ... In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...compatible with the introduction of unique molecular identifiers, which can improve the statistical power of in... of the pRDA_526 vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses...Context-Dependent Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System...

  17. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...some good guidelines to follow in order to ensure that people can quickly and easily identify what your...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

  18. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...citations for older publications. (As an interesting aside, Nature recently reported that the most cited work...their own libraries and offering another way to consider and quantify the impact of scientific publications...find ways to capture scholarly work that exists outside of traditional scientific journals. Of course, ...

  19. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...communication? It is important to identify your audience and consider their background. Social media is...contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your top tips ...communication posts Sign up to receive our science career guide Learn about different careers in science communication...

  20. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...
Showing: 581 - 600 of 902 results