We narrowed to 619 results for: LIS

... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

...grant situation. So, young researchers need to publish good publications in a short period of time and...

...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure...

...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...

...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time...

...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...

...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

... a graphical representation of the results and a list of the analyzed samples (see below).   If the sample...

...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...