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We narrowed to 813 results for: TIM

Showing: 581 - 600 of 813 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

  2. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... a catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use lentiviral...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

  7. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

  8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...interaction is typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...modifying these tools for COVID-19 detection with the ultimate goal to develop low cost and quick point-of-care...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about it ...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

  14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...without the need to find a llama farm! To find an optimally binding sybody, you want to start out with a large...protocol goes through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  18. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...
Showing: 581 - 600 of 813 results