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Showing: 581 - 600 of 797 results

  1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...without the need to find a llama farm! To find an optimally binding sybody, you want to start out with a large...protocol goes through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the...

  5. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

  14. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...
Showing: 581 - 600 of 797 results