We narrowed to 830 results for: ache

...specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target... Will combining the different mutations found in each of these great tools further enhance genome editing...

...expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...

...interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...

...expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ...

...share a common genetic background, which means that each mouse is genetically identical and all mice of the...genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the desired genotype...

...infect more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will ...at increasing scale over the course of 1-2 weeks. Each round of amplification should result in a 10-100...

...teams of three in sections of 15 students. Within each section, teams are assigned different groups of ...research question, the plasmids are also used to teach students about restriction mapping and western blotting...

...yield and higher absorption than it’s predecessor, sREACH, allowing for reduced artifacts due to residual...greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. See Addgene's...

...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...about the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update ...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...

...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible Cas enzymes Are we truly PAM-less? Have we reached an essentially PAM-free solution? Almost! Assuming...

... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...

... that this step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds...generate a reliable antibody for protein tagging approaches” was not part of the goal when Evan’s team generated...

...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

...fluorescence intensity decreased whenever the magnet approached the sample (by less than 1%) and recovered after...MagLOV will be possible with other engineering approaches and that magnetosensitive tools can be developed...

... First, the individual sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid...are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI nucleotide...

...most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a ...specific anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral surveillance by enabling rapid...

...avaliable for less than than it would cost to buy each plasmid in the kit individually. If I order the...more than one gene inserted into a backbone, and each is given its own gene/insert section on the plasmid...

....1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the new...with them and feel the satisfaction of checking off each step as you painstakingly complete your experiment...

... of the university impacts the number of women reaching senior positions. There has been a lot of engagement...considering how important it is. I would really like to reach all those amazing students that want to have a big...