We narrowed to 682 results for: eng

1. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

2. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...and your research community! We work to provide engaging and informative resources that answer questions...

3. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations...

4. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient, and, as...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

6. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...practical use in the following way. Scientists engineered a multiple cloning site (MCS) into the α-peptide...

7. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   Published

   May 22, 2014, 4:02 p.m.

   ...the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript and help...

8. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ...current position Understand your interpersonal challenges if they know some of the people you work with...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...genomes of mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and...

10. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

11. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

12. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

13. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

14. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

17. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

18. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

19. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...