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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 780 results for: ina

Showing: 581 - 600 of 780 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent assay...each sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...exchange occurs through two sequential homologous recombination events: The plasmid carrying the desired genetic...activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa ...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...opportunities available to me as a graduate student culminated in my founding a nonprofit called Central Indiana...skills, and meet new people. There were visiting seminar speakers each week, my department and graduate ...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... of two proteins from a single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes... Melina Fan...

  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ..., such as knockout generation or homologous recombination.  However, experiments that require long-term... laboratory reagents, equipment, and expertise. Final thoughts The Cas9-gRNA RNP method provides scientists...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...actively use tools designed to help reduce or eliminate bias, just like the ones made available through.../s41586-022-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo,...

  10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...possible, unlike methods involving homologous recombination. Additionally, these transposon also exhibit...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... cognate protein. In some cases this property eliminates the need for removing excess dye from the sample..., are photoactivatable, and function in vivo.* Final thoughts The ease of use and continual improvement...

  13. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...grant professional’s job duties can range from coordinating the grant application and submitting it, to ... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

  14. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic... family member, want to train for a triathlon, finally buy that charming fixer-upper house you have always...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...codons, the ribosome may therefore stall at inappropriate locations or fail to make it through the entire...and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression is enhanced...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...team discussions can often lead to otherwise unobtainable solutions. To some extent there is the need ...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual...

  17. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...of previously acquired skills such as speech, coordination, purposeful use of hands, and walking. They ...Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang ...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors unique to this set of tools and...quickly. Beyond the service This brings us to the final piece of the viral vectors service puzzle: the usefulness...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...with qPCR would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...
Showing: 581 - 600 of 780 results