We narrowed to 679 results for: mos

1. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...based on the cloning strategy you followed. While most of the troubleshooting regarding this step has to...

2. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ... AAV2/8 has a type 2 ITR and a type 8 serotype. Almost all vectors contain type 2 ITRs, which comes from...

3. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...with several hypotheses on the cause. To test the most likely cause, you implement an experiment designed...

4. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...write anything good again.’ ” For Susan, one of the most satisfying parts of the job is having completed ...

5. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...proteins in situ immunocytochemistry is perhaps the most common approach. With a good and specific antibody...

6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...CRISPR-Cas9 to function on its target.  They name their most potent Acr-LOV fusion protein inhibitor CASANOVA...

7. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...stripped. HRP-RANbodies are, however, compatible with most multi-labeling immunohistochemical protocols where...

8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel configuration), typical...

9. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...consensus of academic stakeholders. However, while most institutions are indeed going ahead with their planned...

10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ... nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur naturally in...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ... in the field of mouse transgenics. A few of the most common uses are listed below. Cre-dependent gene...

12. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...response programs. NRs also encompass one of the most successful targets for drugs currently available...

13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...glycoprotein (BaEVRless) had high rates of transduction for most cell types they tested. Interested in using Nanoblades...

14. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...When probing multiple targets in parallel, use the most narrowly specific secondary antibodies available...

15. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...Interviewing remotely Remote interviews are likely new to most people - even hiring managers and your interviewers...

16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...store and share, making recombinant antibodies the most practical choice. Hybridomas are much tougher to...

17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...will not result in one complete, circular assembly. Most of the time, these plasmids are returned as one ...

18. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...for finding retinal cell-type specific promoters. Most existing promoters express in a broad number of ...

19. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...simplistic and it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially...

20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...transcription start site will terminate transcription of most of the ncRNA (Figure 1D). While this method can ...