We narrowed to 662 results for: mpo

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent...

2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...plotted against the known protein concentration. Importantly, you’ll want a portion of your standard curve...

3. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies...

4. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...gene is expressed in a particular cell type is important not only for translational research, such as gene...

6. Early Career Researcher Toolbox: Finding Relevant Papers

   Type

   Blog Post

   Published

   Jan. 9, 2020, 1:45 p.m.

   ...and figures using machine learning to extract important features such as influential citations, images...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...multiplexed analysis of cells pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral...

8. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

9. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...at Boston College. National reports stress the importance of providing authentic research experiences to...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...

13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...decide to remain here.  In a time when it is so important for postdocs and graduate students to think about...

14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...belong to. The team at the Victoria Makerspace is composed of computer scientists, engineers, and biologists...

15. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...also have a dedicated app with a social media component, but if it doesn’t seem like this is being used...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...

17. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...production process? These questions are especially important when producing multiple vectors in parallel because...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...