We narrowed to 629 results for: ncl

1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...historical studies of pathogen research have conclusively shown that barrier protocols are vulnerable ...

2. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...

3. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

5. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...

6. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

7. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

10. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

11. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

13. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...

14. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...

15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...assigning a physical location to your samples, include as much detail about that location as possible....

16. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

17. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166...

18. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...whether you realize it or not. These skills might include collaborating, managing, and communicating. After...

19. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ... use of other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google...

20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue...