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  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Viral components typically total <1.5kb, which is rarely more than 30% of the transfer plasmid. Many transfer...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper to use...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and the gRNA...

  6. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...daughter cell during cell division. They don’t simply rely on chance (or random diffusion) to make this happen...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...researchers used the reporter to visualize biologically relevant forces at focal adhesions in living cells in several...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...aurum, M. smegmatis, Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus ...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...conformations and interactions. For difficult to release proteins, including those in the nucleus, more ...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...

  19. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...FRET occurs. By photobleaching the acceptor, you release the donor's quenching and the fluorescence of the...
Showing: 581 - 600 of 617 results