We narrowed to 628 results for: rel
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TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m.... AAV questions every year on a range of issues related to AAV vectors, many from people trying to answer...
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Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....maintaining the structure of your samples, the relationships between cellular components, and the accessibility... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....sites from the 18,385 observed with REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through ... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....around. Finally, purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m.... can be cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....CpG dinucleotides in their 6-mer sequences, are rarely found in the transcriptomes of these species and... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....of the growth factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....antibodies conveniently allow for unlimited production, reliable expression, and easy distribution as DNA (Trimmer... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....set up, conducted and analysed with care to get reliable information. Although two-hybrid systems are a... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....close-out a grant. Grant professionals also build relationships with foundations and government grantmakers.... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... of the reasons why we have only three biology-related Nobel prizes, out of which only one was done in... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....Viral components typically total <1.5kb, which is rarely more than 30% of the transfer plasmid. Many transfer... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....that the desired edit will be performed on all relevant bases within the editing window, for example all... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....conformations and interactions. For difficult to release proteins, including those in the nucleus, more ... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and the gRNA... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....daughter cell during cell division. They don’t simply rely on chance (or random diffusion) to make this happen... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction...