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We narrowed to 871 results for: sta

Showing: 581 - 600 of 871 results

  1. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...you’ve ever been curious about what it takes to start your own nonprofit, this is the episode for you....the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA ...Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene...

  2. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...you with your experiments, but a new look. We started the blog in 2013 to connect with the scientific...dropdown menu. These pages are a great place to start if you’re new to the topic. Or, try the search bar...articles on the Addgene blog by subscribing to instant alerts below.  ...

  3. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...showcase it all!           View this post on Instagram                       A post shared...able to #DeckTheLab and continue the tradition we started last year: The Christmas Decoration Competition.... 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind...

  4. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective...Recommended Reading For those looking to delve deeper or stay tuned, there are plenty of online resources available...available. I recommend a few: -The recently established Ebola Genome Portal at the University of California...

  5. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...Starting today, you may notice some changes to our website! Part of sharing science and making access...plasmids from a specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and...may find one of your most frequently used buttons stands out better on the page, or that the text is just...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ... in some form? There is a lot of talk about the state of scientist training in the US and around the world...postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard. Students also partner with...their own hands, there are some easy ways to get started. I am happy to work with any group of trainees ...

  7. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...Goals of the Center for Regenerative Meidince - Understanding and developing means to treat disease through...surfactant producing cells that can be used to better understand the biology of certain lung diseases. Chapter...Outro - remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to ...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...interest, C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants.... available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA...that they cut at arbitrary sequences a defined distance away from the recognition site. In other words...

  9. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ... after all, with each generation of scientists standing on the shoulders of their colleagues and all those...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science... as much about open access as it does about the state of peer review. 8)     That same issue of Science...

  10. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...infects a snail’s eyestalks. Once ingested by the snail, the larvae reach the eyestalk and transform them...fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert...

  11. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... that purified GFP could be imaged in the green state after excitation at 488 nm, photoconverted with ...with 405 nm light, and then captured in the red state after excitation at 561 nm. This imaging process worked...Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein. Nat Methods...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...sharing as the scientific community strives to understand COVID-19. While the specific motivations vary...scientific community can make progress towards understanding this disease. Figure 1: Scientists are...investigate COVID-19, and once universities reopen and restart other research, scientific sharing will remain ...

  13. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...circulating tumor cells, we found that the binding stability really mattered for efficient capture. We wanted...Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the domain...(CTCs) is going to have a big impact on our understanding and treatment of cancer. It is clear that one...

  14. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his...every fluorescent protein under the sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the...Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted him to deposit...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...specified by the Cas protein These elements were the starting point towards the development of the first cytosine...divisions, indicating that this method produces stable edits. However, this system is also subject to ...cytosines in the base editing window, reducing bystander editing (Wu et al., 2025). Adenine base editors...DNA before the edit is made. Using ABE7.10 as a starting point, Gaudelli evolved the base editor into 40...

  16. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...during the first three weeks that we had made a mistake by not having the right concentration of a reagent...running on a time crunch and knew that if we were to restart the procedure we would not have enough time. During...

  17. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...down the hall who got it from some technician downstairs who got it from who knows where), now is as good...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...

  18. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...work.  5. Do you enjoy bench work? It’s easier to stay at the bench longer (or for your whole career) with...often technical career tracks for PhDs who want to stay at the bench. So if you enjoy wielding a pipette...hopefully the questions in this blog post can help you start to figure out if a master’s is a good fit with your...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ..., does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally...

  20. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...during an initial fixation step. This serves to stabilize DNA-protein interactions for subsequent sheering... of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage Under Targets and Release Using Nuclease...likely that this technique will quickly become a standard method alongside ChIP. Many thanks to our guest...
Showing: 581 - 600 of 871 results