We narrowed to 855 results for: NTS

... and suggest follow up experiments regularly? Can you work on some experiments of your own choosing? Does...scheduled or only ad hoc? Does the mentor keep appointments with group members or are they frequently cancelled...the department? Are they successful at getting grants and funding? Beware of bullying Your mental health...harassment or bullying. Even with the rise of movements such as the Future of Research Symposium and #...develop transferable skills by attending career events or being involved in a grad or postdoc association...there will be a way to grow and learn. Graduate students and postdocs in particular must care about this...

...different points in the pathway. Photoswitches are great for teasing out important branch points along a... to know about them to be successful in your experiments? In this post, we will discuss some broad classes...flexibility in using optogenetic tools in their experiments. Some tools turn on and off rapidly with light...determine which one(s) are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength... light stimulus. This can make or break your experiments!  First, closely read the publication, including...number of factors that can wreak havoc on your experiments, so it is important to understand the details...

... results with reference sequences To design experiments for further plasmid verification Identifying...process for incoming plasmids can vary based on the contents of a plasmid, the first step is always to send...for next generation sequencing. We then copy the contents of the resulting FASTA file into the desktop version.... Using Snapgene for additional verification experiments: Primer and restriction digest design Some regions...These regions require additional verification experiments that can be partially designed using the Snapgene...create custom annotations and design additional experiments like restriction digests. We’ll have a tutorial...

...weeks or every month is long enough that some experiments can get done and data analyzed between each meeting... from the lab members who are conducting the experiments to the PIs. Remote meetings can be tricky, and...communication tools such as Slack. Share data and documents While having multiple scientists edit the same... slower, sequential communication of emailing documents to one person at a time. Just be sure there is...preoccupied with other work or prioritize other experiments. Therefore, it’s crucial that everyone meets ... Some things to figure out are timelines for experiments and manuscripts, who is responsible for which...

...methods and the types of experiments they are commonly used for.  Table 1. Points to Keep in Mind When Performing...Visualize components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...fluorescent protein for single and multi-color imaging experiments. Finally, what kind of system will you use to... Performing Biosensor Experiments Experiment  Detection Method Notes Quantification of a process ...

...for this project, what would be your critical experiments and why? What techniques and skills do you ...another in a way not done before in your field?” He points out to “not be shackled by your own subject - maybe...applied to fungal biology.” Connie Cepko advises students to consider their goal of PhD training when selecting...” Professor George Church encourages graduate students and postdocs to follow their passions and dive...Research projects are designed to train graduate students and postdocs to think analytically and critically...elucidate which project is right for you.   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

...as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et...different parts together. By combining two optimized elements of an antibody probe, scientists can now more ...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (... HA (smHA) which contains a 10xHA tag. These experiments demonstrated that the HA frankenbody can be used...tool-kit at Addgene to get started with your experiments! What else can you do with the HA frankenbody...

...highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden Stopwatch...agreement. Below, you will find some easy talking points for broaching this subject with your institution...professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific examples...against one’s own record Mention Addgene Master Agreements. By default, your TTO must sign an agreement ...respective Master Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering...the processing time of request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest...

...useful for a wide variety of high-throughput experiments. There are currently 390 pooled libraries available...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ...both mouse and human use. Each of these libraries accounts for chromatin, position, and sequence features...designed for all kinds of different high-throughput experiments. Our most popular screening library is the MORF...regulatory sequences ready to be assembled with other elements using golden gate cloning. This toolkit is designed...know which pooled libraries you’ve used in the comments! Resources Additional resources on the Addgene...

...extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and...Wollman and became the widely adopted name for these elements. At the time, the use of episome seemed appropriate...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... vectors that could be used in these cloning experiments. Although plasmids started as a somewhat niche...that can be diversely applied to many different experiments. Addgene was founded in order to store, QC, curate...