We narrowed to 599 results for: REV;
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TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m....need to be signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it ...items. This will give your supervisor a chance to review what you have discussed and add suggestions if ...
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Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m.... lab, or by a previous group. If the plasmid was made by a modifying a vector previously deposited with... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....from 4) - Dunn et al. 2011 provides an in-depth review of quantitative colocalization analysis methods...wavelengths, respectively, are selected (6). These previous blog posts help with the decision of which FP ... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....These benefits of Cas3 do come at a cost — the previously mentioned issues with expressing all Cascade ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m.... et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological systems...exist at all. Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...