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We narrowed to 803 results for: TIM

Showing: 601 - 620 of 803 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

  8. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading... Design of a Western Blot Troubleshooting and Optimizing a Western Blot Resources on Addgene.org Addgene's...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak, Rising τ, and Decay τ of the three sensors... a snapshot of interacting proteins at a certain time and genomic location. TurboID improved on other ...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

  12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

  14. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

  17. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

  19. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

  20. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...
Showing: 601 - 620 of 803 results