...blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including how...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working.... This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin...

...’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused ...opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized programs...point into further STEM exploration.  Further offerings for both STEM teachers and STEM-loving students...

... of interest). Mesophilic microbes are highly preferable (and successful) as host systems since their ...to guess. Think about it like winter tires in different conditions: the more flexible rubber of winter...reagent repositories and the myriad facilities offered by them which help foster a more productive and...

...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin... Note that histone genes frequently have many different variant names, not all of which can be listed ...

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM... part of my job is getting to talk to so many different people! I communicate with customers, software... get software product management internships, preferably on teams that run within the agile framework ...

...that pathways projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

...nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles, the functional...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...constantly reviewing the resources we currently offer to identify gaps and add to them as techniques evolve...

...synthesis, and multifragment assembly methods are preferred. No such restriction applies to deletions; using...common methods for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-... on Addgene.org Browse Our Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find...

...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular... Jennifer Tsang...

...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...the Iron Response Element (IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...difficult for an individual lab to produce - Hybridomas offer an alternative and renewable source of monoclonal... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...

...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...