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...some good guidelines to follow in order to ensure that people can quickly and easily identify what your...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...citations for older publications. (As an interesting aside, Nature recently reported that the most cited work...their own libraries and offering another way to consider and quantify the impact of scientific publications...find ways to capture scholarly work that exists outside of traditional scientific journals. Of course, ...

...puzzle fell into place, as they, taken alongside the insights provided by the studies in Tsien lab, covered...victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of A. victoria there is a ring...1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein could be used...

...communication? It is important to identify your audience and consider their background. Social media is...contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your top tips ...communication posts Sign up to receive our science career guide Learn about different careers in science communication...

...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

...absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit...fixable dye stains that react with free amines on and inside the cells and are thus typically used for distinguishing...vector.) In our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction...should exclude APC-Cy7 from the rest of our panel. Besides some background cell death constantly happening...Compensation Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository...

...antibody, which recognizes the primary antibody and provides the method of detection. You’ll also want to use...is used to detect proteins on the surface of or inside whole cells. Antibodies - typically, primary antibodies...staining. This panel of antibodies is used to both identify the cell subsets of interest from among the mixture...This could be cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture...IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978...

...Cre - A useful tool for neuroscientists AAVs are widely used by neuroscientists because they efficiently...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. For example...only be expressed in neurons. Indeed, this AAV provides you with a means to specifically activate expression... viral vector blog posts for many more tips and ideas on how to use these fantastic tools in your experiments...

.... Although my interests are very clear, I had no idea how to translate them into real world careers. I...this new virtual world, I was worried about how my ideas would be translated through Zoom calls and Google...frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for what my Instagram...with Quintin Marcelino who is Addgene’s Science Video Production Specialist and Arthur Guerra who is a...

...Stacey from Eastern Tennessee State University and provides a brief rundown of Sean’s top five smartphone ... lab life a bit easier. Since the post doesn’t provide an exhaustive list of all the apps out there, we...reasons: 1) I think phage are super cool and have considered getting a tattoo of my favorite molecular biology...to screen for proteins that could be useful for a wide variety of reasons. 3) I would love to see more ...

... but if it is not possible consider a phone or video call. If you do decide to use Skype, make sure to... from other scientists doing interesting work. Consider this to be like any other research project. You...

...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open science, ensure ...MOOC), with an explosion of courses and content providers and participation from many well-respected and...

...them remain uncharacterized. This represents a considerable barrier to furthering research and developing... an assortment of genes. Using patient data to identify mutations of unknown importance in cancer Previously... Center patient databases, this research team identified 1,049 perturbations in several genes spanning... 33 cancer types. The types of mutations they identified included gene amplifications and fusions, missense...

...Maureen Hanson’s and Rima Menassa's labs made the accidental discovery that laser treatment can photoconvert... Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible fluorescent...great scientific discoveries, this one was made by accident. After exciting GFP-labeled plant cells at 405...variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent proteins for your imaging...

...spoke with Daniel about the interplay of these two ideas he said “science is a form of art to me… The problem...to have increased value and is appreciated by a wider audience for more than just its biological importance...of familiar flowers." This new x-ray technique provides scientist with a new method to explore the inner...organisms without destroying the original, which could provide a new tool to study old and unique samples, such...

...succesful scientists have time for fun and life outside of the lab? This final post in the PI Interview...other. The Church Lab enjoys each other’s company outside of the lab by sharing meals, going on trips, and...school year winds down, take some time to have fun outside the lab! How does your lab celebrate each other's... fun outside the lab...

...base editor, BE3, induces a low frequency of genome-wide off-target DNA editing. Using several rapid and ...-effective screening methods, David Liu’s lab identified and validated CBEs with reduced Cas9-independent... tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing by TET1 by co-delivering...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university...anti-tech or pragmatic.” Advice for pursuing careers outside of academia Recently, graduate students and postdocs...skills that are applicable to a variety of careers outside of academia. She has had previous graduate students...

... poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty quickly notice...fluorescent proteins to observe what’s happening inside cells for at least a couple of decades. Green is...jellyfishes and corals, but all of them fluoresce outside the transparency window of mammalian tissues,” ...capable of revealing, for example, how muscles work inside a living animal. For those making use of these ...

...CAST, the nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and ...for a variety of loss-of-function mutants could provide a streamlined, one-size-fits-most approach. evoCAST...Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Nature, 571(7764), 219–225. https...Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...