We narrowed to 839 results for: LEA
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TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....plasmids from this study with Addgene. Read on to learn more about their work, and how these plasmids can...PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read all cancer...
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Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....publication. As you develop and confirm new gRNAs, please consider submitting their sequences (and plasmids...press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR Software ... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....hoped to use Twitter professionally to network, learn more about alternative careers for scientists, and...to parity - the smaller the number, the more it leans towards males). This ratio is more skewed towards... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....at a frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system.... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....National Academy of Sciences in March, he and his colleagues took another important step forward by dissecting...our understanding and treatment of cancer. It is clear that one needs to capture a diverse range of CTCs... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....manipulation tools Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze.... Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker can help you ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....targeted RNA knockdown, which leads to unique non-specific RNA cleavage in bacteria. In mammalian systems... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....for scientists to understand how GPCR activation leads to downstream effects. Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....issues as one doing experiments in France. We can learn with each other through globalized, collaborative... Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your Research 5 Reasons... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....last, homework assignments. The instructors are leaders in the field of microbiology with multiple high-profile...groups, we were required to critique two of our colleagues’ manuscripts and discuss changes to particular... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask – leave a message in the comments below and an Addgenie ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....against the variants? Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard...Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....ever like to write about a recent plasmid deposit please sign up here. Human whole genome sgRNA iBAR ...calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase that...