We narrowed to 845 results for: LEA
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TypeBlog PostPublishedDec. 30, 2015, 3:30 p.m.... human cells are avaliable for distribution. To learn more about pooled library screening, read our recent...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some...
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Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....selectively kills only the bacteria you want it to, leaving all those healthy bugs in the human microbiome ...graduate student in Feng Zhang's lab, in a press release. "In the future, it could allow us to design versions... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....one is right for you, so if you’re interested in learning more about fluorescent proteins, I invite you ...you to download our Fluorescent Protein eBook and learn much more! ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....number of out of office responses I’ve received). Clearly we have all of our writers to thank for the excellent...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....plasmids from this study with Addgene. Read on to learn more about their work, and how these plasmids can...PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read all cancer... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....publication. As you develop and confirm new gRNAs, please consider submitting their sequences (and plasmids...press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR Software ... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....hoped to use Twitter professionally to network, learn more about alternative careers for scientists, and...to parity - the smaller the number, the more it leans towards males). This ratio is more skewed towards... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....at a frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system.... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....National Academy of Sciences in March, he and his colleagues took another important step forward by dissecting...our understanding and treatment of cancer. It is clear that one needs to capture a diverse range of CTCs... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....for scientists to understand how GPCR activation leads to downstream effects. Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....targeted RNA knockdown, which leads to unique non-specific RNA cleavage in bacteria. In mammalian systems... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....manipulation tools Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze.... Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker can help you ... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....issues as one doing experiments in France. We can learn with each other through globalized, collaborative... Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your Research 5 Reasons... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....last, homework assignments. The instructors are leaders in the field of microbiology with multiple high-profile...groups, we were required to critique two of our colleagues’ manuscripts and discuss changes to particular... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now...