We narrowed to 616 results for: LIS

1. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...audience? As Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...

3. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...

4. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...

5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ....0, with the goal of overcoming the limitations listed above. First, they screened a variety of fluorescent...

6. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ... H., Dumitras, C., Gong, B., Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J...

7. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021...

8. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...

9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ...plating, etc. Be wise to choose any of the techniques listed below and always use a second assay to be 100% ...

10. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...connect to your why. I suggest this crowdsourced list of foundational texts as a starting point.  Assess...

11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

12. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

13. Deep Dive: Statistical Tests (Comparisons)

    Type

    Blog Post

    Published

    March 7, 2023, 2:15 p.m.

    ... of comparisons. The Bonferroni does this by establishing a significance threshold at a p value less than...

14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...doing routine PCR assays. Here is a simple to-do-list to follow when planning epitope tagging experiments...

15. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...manufacturers. It also includes a rough comparison of list price between these manufacturers, as price is a...

16. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ... the forward or the reverse primer.  There is a list of useful primer design resources in the References...