We narrowed to 711 results for: Met

1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome editing. For more...

2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

   Type

   Blog Post

   Published

   Nov. 1, 2017, 2:04 p.m.

   ...proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think this helped...

3. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...through our hot plasmids articles. Think we missed something hot? You can pitch a Hot Plasmids section (antibodies...

4. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   Published

   July 7, 2016, 2:30 p.m.

   ...Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn how to Network...

5. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   Published

   March 31, 2015, 4:08 p.m.

   ...collaborating effectively with other teams. While they sometimes dig in and do some of the work, they must be more...

6. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “punish” someone...

7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

   Type

   Blog Post

   Published

   Jan. 15, 2015, 1:50 p.m.

   ...modifying, and improving the CRISPR-Cas9 system. Mehmet Fatih Bolukbasi from Scot Wolfe's lab described...

8. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...in fly culture cells like S2 and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There...

9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

   Type

   Blog Post

   Published

   Sept. 21, 2023, 1:15 p.m.

   ...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...

10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ...fact: A 1 cm block of polymer can generate many meters of fibers!     Fig. 1 - Overview of the...

11. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

    Published

    March 28, 2022, 4:30 p.m.

    ...through Rigor and Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j....

12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ..., Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (2012) Remarkable stability of an instability-prone...

13. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    Published

    Dec. 21, 2017, 2:06 p.m.

    ... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...

14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392. PubMed...

15. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    Published

    March 26, 2019, 1:40 p.m.

    ...inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been limited. Like...

16. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type

    Blog Post

    Published

    Nov. 8, 2018, 3:47 p.m.

    ...genes where there are instances of rare codons, sometimes intentionally modulating expression in ways we...

17. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    Published

    Sept. 18, 2018, 1:33 p.m.

    ...cells through fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that...

18. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, ...

19. Plasmids 101: Origin of Replication

    Type

    Blog Post

    Published

    Nov. 11, 2020, 2:23 p.m.

    ...started to die off. Have any questions? Need something clarified? If so please, post in the comments....

20. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ...like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March...