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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 850 results for: NTS

Showing: 601 - 620 of 850 results

  1. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...discussions are becoming a ubiquitous format for events and sessions in scientific conferences. They can...a table. It adds a formality and distance that prevents interaction with each other and the audience. ... inviting interesting friends and colleagues to events for this purpose. If possible, make sure the panelists...

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...difficult to ensure comparable expression of all components of Cascade for maximum efficiency of the process...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two... mentioned issues with expressing all Cascade components can make Cas9 easier to work with than Cas3. ...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... antibodies for aminoacyl tRNA synthetases Nanocompartments for yeast bioproduction pathways The CRISPR...with previous research, which required protein compartments to co-localize multiple enzymes in a reaction...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...find more detailed information about the plasmid contents? The middle section of our plasmid pages contain...information you'll need to use the plasmid in your experiments: Backbone: The starting vector that the depositing...from Addgene!).  It also contains links to any agreements under which the plasmid is distributed.  All ...

  6. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...CRISPR affinity purification in situ of regulatory elements (CAPTURE) (Liu et al., 2017). This system utilizes...techniques, this method also has great potential for improvements and adaptations that can provide an increased..."Efficient sequence‐specific isolation of DNA fragments and chromatin by in vitro enCh IP technology using...

  7. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...exception to this effect -- meticulously designed experiments in labs around the world were put on hold indefinitely...the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential...corresponding factor 𝜙z if t is during event z. The "events" we considered for our model were weekends, Christmas...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...present in the HEK293T cell line used (which have fragments of the AdV genome in them, including the E1 region...intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and adaptive immune ...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... that introduced an ancestry bias into their experiments instead of a true correlation.   It turned out...five-team response to address this issue, but it still points to a far larger and more complex problem endemic...contributes to false negatives in CRISPR-based experiments with varying burden across ancestries. Sean A...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...remember where you read particular studies or experiments. Later, you can easily convert the citation style...much you can get done between office hours and experiments. A comment about citing: I wasn’t sure if I should... be revolutionary, but you could include a few comments on where you think the field is going or what ...

  11. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...regularly spaced denticles (bristles) outline the segments of the Drosophila embryo. In flies with non-functional...1997). We have a lot of plasmids that contain components of the SB transposon system including those from...of this gene name but the larval gut of bagpipe mutants vaguely resemble a bagpipe and historically, bagpipes...

  12. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...what does this mean for a molecular biologist who wants to express genes from one organism in another? Let...organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson et al....optimization should not be used in some in vivo experiments and in biotechnology and therapeutics (Mauro ...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit plasmid replication...The concentration of negative regulation system elements in the cell is artificially high for both plasmids... uncommon, or perhaps a synthetic biologist who wants to develop a plasmid-based tool that is easy to ...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as...system that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern ...-well plate basis, but for rapid testing and refinements, we specified a select small number of input ...

  15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...silencing required addition of chromatic-opening agents to boost expression to appreciable levels. We wanted...expression and robustness without chromatin-opening agents. It ended up being very straightforward to engineer...for improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via ...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...selective pressure is necessary or for long-term experiments. They’ve also helped researchers design and optimize...

  17. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...making them more difficult to treat in the future. Patients not finishing the whole course of antibiotics ... can utilize tactics like rotating antibiotic treatments, so that exposure to each antibiotic is minimized...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...rounded out the top four average video abstract view counts.” For researchers looking to garner more attention...can be shared by research scientists, faculty, students, private sector researchers, or pretty much anyone...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...

  19. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...sequential homologous recombination events, but because these events are rare, each requires selection....efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization...

  20. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs...parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized plasmid....template). Due to the small difference in size, the fragments should be separated on a high percentage agarose...
Showing: 601 - 620 of 850 results