We narrowed to 813 results for: TIM

...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

...like FRET, BiFC experiments must be carefully optimized to be successful. A BiFC experiment generally ...inflammation and neurodegeneration. He now spends his time learning and writing about science whenever he's...

...can try re-purifying the sample you have, but if time and resources allow, you might be better off starting...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, of course, ...

...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

...disrupted. As our ESCs die and differentiate over time, we periodically use next-generation sequencing ...CRISPR. Therefore if a gRNA drops or disappears over time, we infer that the enhancer it targets is a “pillar...

... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

... pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

...such as the brightness, photostability, maturation time, and acid sensitivity of various fluorescent proteins...References Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation...

...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

...again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading... Design of a Western Blot Troubleshooting and Optimizing a Western Blot Resources on Addgene.org Addgene's...

...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak, Rising τ, and Decay τ of the three sensors... a snapshot of interacting proteins at a certain time and genomic location. TurboID improved on other ...

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...