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We narrowed to 813 results for: TIM

Showing: 601 - 620 of 813 results

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

  8. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  9. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

  12. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

  13. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading... Design of a Western Blot Troubleshooting and Optimizing a Western Blot Resources on Addgene.org Addgene's...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

  17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak, Rising τ, and Decay τ of the three sensors... a snapshot of interacting proteins at a certain time and genomic location. TurboID improved on other ...

  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...
Showing: 601 - 620 of 813 results