We narrowed to 830 results for: ache

...fluorescent protein Excitation & Emission (ex/em): Each FP has its unique ex/em peak. Therefore, choose ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...

... great promise in preclinical studies and could reach clinical trials soon. The AAV safety profile is...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing...

... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science...

...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....it into sticking to the hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and...

...organization with a different person responsible for each figure. Others still are more casual with a single... applications and should be carefully chosen for each group and topic. For example, it may be useful to...

...genes and which applications are best (or worst) for each antibiotic. The use of antibiotic resistance DNA...a look for a quick recap of the pros and cons of each antibiotic and happy selecting!   Antibiotic...

...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce...

...cells stay in an HDR-promoted cycle longer. This approach may have some negative outcomes on viability if... as 10 hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that...

...platforms.  Algorithms can make it difficult to reach people who are not already actively interested in...  Rachel Leeson...

...culture inoculated from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5...very important to select multiple T1 plants from each transformation. I like to isolate a minimum of five...

...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...substantially identified more hits than the SAM library approach in a comparison study screening for vemurafenib...

..., Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express up to 4 ...LbCpf1 (blue). Mismatch positions are indicated below each graph. Figure from Kleinstiver et al., 2016. ...

...Although not listed here, many permutations within each color also exist with only slight variations separating...excitation/emission spectra are used; one fused to each protein or domain being tested. Find FRET plasmids...

.../Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological...Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen design.   Bassik...

...re likely to find current job openings. A good approach is to do this first for companies at which you...originally called about, you can keep in touch and reach back out when you are looking to change positions...

... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

...combined with different fluorescent reporters for each enzyme. In addition, Csm6, a CRISPR type-III effector...Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti...

...measure the target. However, the two different approaches allow for optimized strategies for differing ...specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must be carefully...

...withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar and Sandoval... can bind and transduce many cell types before reaching the target cells. VSV-G is also toxic at high ...

...composed of actin and myosin which interact with each other to generate forces that cause cells and muscles...and historically, bagpipes were made using the stomachs of different animals. Check out the Addgene website...