We narrowed to 662 results for: mpo

1. 3D Printing Meets CRISPR Cas9

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   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

3. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...production process? These questions are especially important when producing multiple vectors in parallel because...

4. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

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   Sept. 7, 2017, 12:17 p.m.

   ...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

5. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...

6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to test them all. ...

7. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...respondents (transfection method, amount of each component per transfection etc.). Take Pooran's NgAgo Survey...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...native gels fail to detect EGFP dimerization. Importantly, all of these assays are performed in vitro. ...

9. Light Sheet Fluorescence Microscopy

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   Blog Post

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   July 16, 2015, 2:30 p.m.

   ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

10. Plasmids 101: Multicistronic Vectors

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    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...expression levels between two or more genes is important. How do multicistronic vectors work? Scientists...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and ...

12. Which Fluorescent Protein Should I Use?

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    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

13. Simplify Cloning with in vivo Assembly

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    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...cloning strain has failed so far. What is most important is the transformation efficiency of the strain...

14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... enough for efficient library performance, an important criterion for probing a limited number of cells...

15. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...

16. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...this technique, researchers have begun to make important discoveries in various biological fields. In 2015...

17. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ...design of the experiment or dataset itself. It is important for scientists to not only be aware of the potential...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ...purification protocol uses phenol, then it is important to confirm that your sample’s absorption peak ...

19. Great Results Start with Great Standard Curves

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    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...

20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...excess dye from the sample, which is particularly important for samples where washing is difficult, such as...