...copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit plasmid replication...The concentration of negative regulation system elements in the cell is artificially high for both plasmids... uncommon, or perhaps a synthetic biologist who wants to develop a plasmid-based tool that is easy to ...

...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...selective pressure is necessary or for long-term experiments. They’ve also helped researchers design and optimize...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as...system that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern ...-well plate basis, but for rapid testing and refinements, we specified a select small number of input ...

...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...making them more difficult to treat in the future. Patients not finishing the whole course of antibiotics ... can utilize tactics like rotating antibiotic treatments, so that exposure to each antibiotic is minimized...

...rounded out the top four average video abstract view counts.” For researchers looking to garner more attention...can be shared by research scientists, faculty, students, private sector researchers, or pretty much anyone...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...

...sequential homologous recombination events, but because these events are rare, each requires selection....efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization...

...remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs...parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized plasmid....template). Due to the small difference in size, the fragments should be separated on a high percentage agarose...

..., ligases (notably T4 DNA ligase) are critical reagents for many molecular biology protocols, including...substrates containing no gaps, and will not join any fragments end-to-end without the exo/polymerase generation...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...

...the march for science on April 22, 2017. While experiments (and editors) can move slowly, news about possible...set up with various science demonstrations, and students could walk around and ask scientists questions... how planets form around young stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com...

...reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization Once generating...of reprogramming, and 2) studies with clinical endpoints. In the first scenario, robust and efficient generation...Let us know about your iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru ...

...decreased immunological markers of stress in adolescents. It also put those young people in a better mood...list? Let us know by sharing it with us in the comments.   ...

...can save researchers time by quickly relaying key points about an unfamiliar topic or lab process. Along...you don’t see here? Give us suggestions in the comments section below! Additional Resources on the Addgene...

...conversations with PIs, lab managers, and grad students. We’ve talked about their hot-off-the-presses ...Investigator. How-to guides for troubleshooting your experiments and getting better results at the bench, with...

...how they were created, and how to use them in experiments. Best practices!). Making the find plasmids ...even easier and more useful, let us know in the comments. I hope this helps! Additional Resources on the...

...as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been following a set of clues ...yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend We need your help...

... vector services allow scientists to conduct experiments faster, skipping the preparation steps of production... serotype, and prep size they need for their experiments — and we’ve been able to ship them out in record...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...

...four of the key figures Number of requests per reagents (as a horizontal bar chart) Number of requests...requestors, year of first reagent sent, year with most reagents sent, and the most recent full calendar year ...

...without spending time and resources in preparative experiments.  We’re excited to begin offering this service...of Tris buffer suitable for molecular biology experiments such as cloning, digestions, PCR etc. and/or ...

...MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We hope everyone...the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6...