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...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...during the first three weeks that we had made a mistake by not having the right concentration of a reagent...running on a time crunch and knew that if we were to restart the procedure we would not have enough time. During...

...slight detour, and a bit of luck. This journey started not at the end of her PhD but rather at the beginning...upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job search sites...Iowa really opened the door for me at eScience.” Stay tuned for part two of Caitlin’s interview where ...

..., does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally...

...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...down the hall who got it from some technician downstairs who got it from who knows where), now is as good...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...

...work.  5. Do you enjoy bench work? It’s easier to stay at the bench longer (or for your whole career) with...often technical career tracks for PhDs who want to stay at the bench. So if you enjoy wielding a pipette...hopefully the questions in this blog post can help you start to figure out if a master’s is a good fit with your...

...of the societies within a university set up stalls or stands on campus and recruit students to join their...-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology...

...high processivity enzymes and the structural understanding thereof have the potential to provide improvements...conventional antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic yet are still...telomere, and hope it can be used to expand our understanding of proteome localization in future studies of...

... the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see plasmid map and ...dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our proteins that...proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t find an all-in-one...

..., most of the nucleic acid transfected will not stably integrate (unless you used a transposon system,... think. We have protocols available to get you started and information on the specific viruses available... Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage...

...alone.  And a longstanding inclination to be a teacher was looking for an outlet. I started asking people... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...

...biology tool. These systems allow researchers to understand bacterial gene clusters that produce useful natural...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing...

...Addgene prides itself on being financially self-sustaining through distributions of research materials, ...Bradbury A, Plückthun A (2015) Reproducibility: Standardize antibodies used in research. Nature 518:27–29...AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS...

...limitations because it turns a dynamic process into a static measurement and it provides limited information...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...in acidic environments, while mRFP is relatively stable and fluorescences even at the acidic pH found in...

...the 3rd edition has now been released), and have started providing recorded interviews with Addgene community...and actionable content in the coming years as we stay on top of the many developments in science and the...give researchers the background they need to get started using plasmids and the revolutionary CRISPR technology...

...about biology in high school, it wasn’t until I started working in my first molecular biology lab in college...into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds... 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...

...help me get the engagement I wanted. I therefore started planning a game that would force my students to...and strategy skills to apply those concepts and understand how therapeutic strategies work. Find Addgene...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

.... In her graduate work in Michael Lin’s lab at Stanford, Xin Zhou greatly expanded that list by using ...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...throughout her training and wants to make her work understandable to people both inside and outside the scientific...

...hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain from The Jackson...Laboratory. Originally developed and characterized by Stanley Perlman, of the Carver College of Medicine and ...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

...such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. Create yellow...modified form of the cytosolic C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers...PMCID: PMC4469972 Marvin, Jonathan S., et al. “Stability, affinity and chromatic variants of the glutamate...

... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...vitro and in living organisms to better study and understand these diseases and disorders. You can find the...