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We narrowed to 874 results for: sta

Showing: 601 - 620 of 874 results

  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ..., does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally...

  2. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...during the first three weeks that we had made a mistake by not having the right concentration of a reagent...running on a time crunch and knew that if we were to restart the procedure we would not have enough time. During...

  3. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...down the hall who got it from some technician downstairs who got it from who knows where), now is as good...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...

  4. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...work.  5. Do you enjoy bench work? It’s easier to stay at the bench longer (or for your whole career) with...often technical career tracks for PhDs who want to stay at the bench. So if you enjoy wielding a pipette...hopefully the questions in this blog post can help you start to figure out if a master’s is a good fit with your...

  5. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...of the societies within a university set up stalls or stands on campus and recruit students to join their...-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology...

  6. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...high processivity enzymes and the structural understanding thereof have the potential to provide improvements...conventional antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic yet are still...telomere, and hope it can be used to expand our understanding of proteome localization in future studies of...

  7. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ... the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see plasmid map and ...dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our proteins that...proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t find an all-in-one...

  8. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...alone.  And a longstanding inclination to be a teacher was looking for an outlet. I started asking people... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...limitations because it turns a dynamic process into a static measurement and it provides limited information...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...in acidic environments, while mRFP is relatively stable and fluorescences even at the acidic pH found in...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...biology tool. These systems allow researchers to understand bacterial gene clusters that produce useful natural...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ..., most of the nucleic acid transfected will not stably integrate (unless you used a transposon system,... think. We have protocols available to get you started and information on the specific viruses available... Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...the 3rd edition has now been released), and have started providing recorded interviews with Addgene community...and actionable content in the coming years as we stay on top of the many developments in science and the...give researchers the background they need to get started using plasmids and the revolutionary CRISPR technology...

  14. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...about biology in high school, it wasn’t until I started working in my first molecular biology lab in college...into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds... 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...help me get the engagement I wanted. I therefore started planning a game that would force my students to...and strategy skills to apply those concepts and understand how therapeutic strategies work. Find Addgene...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

  16. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    .... In her graduate work in Michael Lin’s lab at Stanford, Xin Zhou greatly expanded that list by using ...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...throughout her training and wants to make her work understandable to people both inside and outside the scientific...

  18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...vitro and in living organisms to better study and understand these diseases and disorders. You can find the...

  19. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain from The Jackson...Laboratory. Originally developed and characterized by Stanley Perlman, of the Carver College of Medicine and ...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

  20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. Create yellow...modified form of the cytosolic C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers...PMCID: PMC4469972 Marvin, Jonathan S., et al. “Stability, affinity and chromatic variants of the glutamate...
Showing: 601 - 620 of 874 results