We narrowed to 855 results for: NTS

...funding and space in academic research labs has more students looking for other options. While graduate programs...academic career development, it's often left to students to identify and build the necessary skills for...research from a broader viewpoint may expose which experiments are crucial in demonstrating the true innovation...entering the workforce increases, EAs allow graduate students and postdocs an opportunity to tailor their career...interests, and skills? Curious to know where graduate students and postdocs are currently getting hired? Let ...more about next. Leave your suggestions in the comments section.  ...

...viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean...from workspaces. Speed: AAV transduction experiments are relatively quick. We have found that expression...benefits make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting ...complexes are so robustly expressed that anatomical assessments can be easily performed too. While I implied ...transport of AAVs is a potential problem in many experiments, a group of researchers has identified this feature...used to treat disease in humans and additional improvements are being made to make them even more useful...Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV...

... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...

...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...known as a *library*. Parallel sequencing of the fragments within a library allows researchers to reconstruct...together to create a single library containing fragments carrying sample-specific barcodes. Rather than...is an author of 20+ scientific publications and patents.   ...

...new NEB kit includes a step that both fragments the DNA and prevents it from forming concatemers later. ...services and expertise without breaking their bank accounts. When I heard about how LabCentral aims to enable...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start of the workshop...itself. This was great because it forced workshop participants from different biotech companies to interact...

... important to know what kind of reagents you work with in experiments. Hence, I’m including this MSDS ...do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made by New England...AMINO can be beneficial for new researchers or students beginning to learn about biochemistry. The least...have a favorite lab app, please share it in the comments section below! Many thanks to our guest blogger...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional Resources on the Addgene Blog...

...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,...them manageable. At Addgene, teams can add as many points as they like to the written agenda, but each only...and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives everyone....  During the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen...for talking, not reading: Send around relevant documents, like proposals to review or project plans, before...send around a Google doc where people can make comments. At meetings, we just focus on the parts where...

...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due to.... A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor ...

...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples of factors...HSF-1), which subsequently binds to heat shock elements in the promoter, thereby activating transcription...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...

... to use the Gal4/UAS system, but the available reagents do not match your needs. If YFG is thrillingly...generate a transgenic fly There are a few important points to consider when choosing the vector you’d like...comparisons between lines expressing different variants of YFG because the transgenes are found in the...won’t be positioning effects). When designing experiments where you want to express multiple transgenes...chromosome your docking site is located). This amounts to a small difference of two or three weeks. 3....for. Making your transgenic fly and downstream experiments Once your gene of interest is cloned into a plasmid...