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We narrowed to 895 results for: Ide

Showing: 621 - 640 of 895 results

  1. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...their work.  Check out Joanne's Reddit AMA   Guidelines for giving feedback Use formal and informal ...real change is possible in your eyes so their confidence is restored. A good manager does not hold grudges...best? What 3 things worked least well? Manager provides feedback (may be the same as self-assessment or...about real performance issues. Watch this little video to learn more about indirect language from Steven...

  2. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...hybridomas) that secrete antibodies in culture and provide renewable reagents with reliable and reproducible...first NIH grant that Addgene has received. Addgene prides itself on being financially self-sustaining through..., Addgene’s interim Executive Director. “NABOR provides a new model for the creation and acquisition of... enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been funding laboratories...

  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...know all about antibiotic resistance genes, let’s consider another basic element of any plasmid: the origin... intend to use, and whether or not you need to consider your plasmid's compatibility with one or more ...copy number varies. See below for additional considerations. *Requires pir gene for replication (reference... external factors contribute as well. These considerations are especially useful to keep in mind if you...

  4. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Addgene closed for almost two weeks and with labs worldwide closing for unknown amounts of time, we were facing... enhances productive collaborations as well as provides visibility for grant-driven research being done...each of many grantors supporting research could provide Addgene with a small amount of funds, the community...and projects to serve the needs of scientists worldwide with Addgene’s traditional excellence through ...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...what gets packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV vector...the WPRE. TL;DR? Watch our AAV Transfer Plasmid video!   Now you’ve got a basic understanding of some...Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

  6. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer Agreement...quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after all, they...unprocessed agreements slow down your research; provide specific examples if possible Tell them about the...exchange of intellectual property (as inventions, ideas, plasmids) among universities and other research...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...dynamic process into a static measurement and it provides limited information about what materials or organelles...autophagic activity. The aim of this post is to provide an overview of four autophagy biosensors currently...protein plasmids and resources Fluorescent protein guide: Biosensors ...

  8. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...attention and enjoy anecdotal forms of information — identifying with a character, building emotional investment... do and should tell stories all the time. They provide accounts of their research struggles in papers.... a weekly lab meeting as you’ll need to for an outsider. And you won’t need to dive as deep into your ...preprint servers, share your email address far and wide, keep your website up to date, invite people to ...

  9. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...explaining a technical piece of research to others outside that specialty, who may be other scientists, or...journalists, or policy makers. The term editor covers a wide variety of careers and jobs. At Science, which is...financially critical. Those who work on the publishing side of science have professional meetings, societies...in the science communications world, you might consider an internship or a short program to build skills...

  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Taylor, 2014). They’re so simple that we can’t even decide if they’re alive or not. Yet these simple, small...Virus structure While all viruses have a genome inside a protein shell known as a capsid, some also have...march to the beat of their own drum. They have a wide variety of possible genome types that can be either...copies, which are released from the cell like baby spiders from an egg sac. (Okay, some enveloped viruses ...

  11. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ... the literature and find an ideal antibody against Protein A and an ideal antibody against Protein B. ...specificity to the target while constant regions are identical for antibodies within certain classification groups...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...

  12. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially ...distribution of cell lines from repositories on opposite sides of the world shows that science is truly a global...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ...15562-9 Subbarao K, Roberts A (2006) Is there an ideal animal model for SARS? Trends in Microbiology 14...

  13. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing tool. However, Cas9’s endonuclease...when studying neuronal activity. The researchers identified MerMAIDS from their analysis of different publicly...comprised of thousands of loops. These loops not only provide a 3-D structure for packing genomic DNA into nuclei...

  14. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...fusion protein, etc...).  There are many online guides – including an excellent series of posts by Joachim... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary...for, the Table of Fluorescent Protein Properties provides a master list of all proteins in the database,...try out the site and contact us if you have any ideas or requests.  Or, if you have experience in Python...

  15. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...performed with embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing...cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat...circulation of following FAP-TAP light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages...Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other...

  16. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...that we could discuss our projects and ideas every day and decide on the best way to structure roles and...-group meetings to discuss day-to-day progress, ideas, and ways to improve the lab. “An effective working...including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and New Faculty...

  17. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...addgene.org Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...to upregulation or downregulation are easier to identify by comparing peak positions to the base expression...2. Quantification using MFI makes it easier to identify and study these subtle shifts. Additionally, you...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works...up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...ll need to ensure you have a bead-only control alongside your input control. Elute the DNA after washing...you generate your data. However, it may be a good idea to add "patience and an extra-methodical approach...

  19. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...this work? Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on ...deaminase, TadA-7.10 (Gaudelli et al., 2017). The idea was to evolve a TadA-7.10 version with a faster ...enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold faster than TadA... base editors for your research Read our CRISPR guide Find plasmids for your research ...

  20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...researchers.There is both theoretical and empirical evidence (in psychology, cancer biology, pre-clinical life...) do you really appreciate how few details are provided in most published papers. The precise timing, ...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity in study...
Showing: 621 - 640 of 895 results