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...the same myths and misconceptions fifteen years later. What does that look like?  One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...

...trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...

...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

...provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as...

...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

...when Gal4 is expressed. Gal4 expression can be regulated by any chosen promoter, and Bloomington’s Stock...make your own. In my next post, I’ll explain the relatively straightforward process of generating your own...

...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph...

...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...

...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

...pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting...

...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an...

...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...

...more credible than they actually are. Throwing out plates that don’t respond as expected, use of representative...different results. Different setting, different population, new genetic variation, or different handling...

...in the native virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used... D. V. (2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10,...

...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows you...

...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

... determine the implications of your genetic manipulations. You can produce some very beautiful immunofluorescent...model organism is in its amenability to genetic manipulation.  This is just a primer so that you are aware...

...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...