We narrowed to 853 results for: LEA

...for scientists to understand how GPCR activation leads to downstream effects.  Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated...

...also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources...

...work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive...

...repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now...

...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and an Addgenie ...

...cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your...

...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux...

...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ...

...against the variants? Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard...Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org...

...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids...

...ever like to write about a recent plasmid deposit please sign up here.  Human whole genome sgRNA iBAR ...calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase that...

...Association and it was through this connection that she learned more about Addgene. “The mission to share critical...and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has...

...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur...

...Plasmids 101 Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR...

... quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...

...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...

...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR...

... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe...

...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ...