We narrowed to 729 results for: NSI;

1. The Golden Stopwatch Award: Recognizing Facilitators of Research

   Type

   Blog Post

   Published

   Sept. 5, 2019, 1:06 p.m.

   ...quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after all, they...

2. Single-cell tracking of lineage and identity with CellTag

   Type

   Blog Post

   Published

   May 9, 2019, 1:15 p.m.

   ...labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6 bp fixed region...

3. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...of fusions and FRET reporters. mTFP1 is also insensitive to physiologically relevant changes in pH. Find...

4. New and Upcoming Viral Vectors - Spring 2019

   Type

   Blog Post

   Published

   March 26, 2019, 1:40 p.m.

   ...calcium sensors are single-wavelength sensors with intensities that change in response to calcium binding. There...

5. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

   Type

   Blog Post

   Published

   Nov. 8, 2018, 3:47 p.m.

   ...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...

6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   Published

   Aug. 11, 2020, 1:15 p.m.

   ...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

7. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials, is now offered ...

8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

   Type

   Blog Post

   Published

   Sept. 21, 2023, 1:15 p.m.

   ...directly conjugated primary antibodies can be expensive and may not provide enough signal to visualize...

9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...Virus structure While all viruses have a genome inside a protein shell known as a capsid, some also have...

10. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...

11. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...hydroxyl group! You may want to include extra extension time after the last cycle of PCR to make sure ...

12. Summer SciComm: Science Storytelling

    Type

    Blog Post

    Published

    Sept. 16, 2022, 1:15 p.m.

    ...need to be overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward...

13. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...HDR, which could lower HDR efficiency. Higher sensitivity to off-targets More research is needed to compare...

14. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...   The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the ...

15. Addgene's Top 10 Blog Posts of 2015

    Type

    Blog Post

    Published

    Dec. 7, 2015, 6 p.m.

    ...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...post has helped clarify some of the important considerations when using, amplifying, and verifying pooled...

18. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ...into a specific bacterium, it can be important to consider the type of bacteria it is being used in, and ...

19. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ...we call “asymmetric collaborations.” These are transient partnerships where a researcher makes a small ...

20. With Our New Viral Service, We're Taking CRISPR Further!

    Type

    Blog Post

    Published

    Dec. 6, 2016, 3:30 p.m.

    ...requested and incredibly useful tools, they require extensive background work from the requesting scientist ...