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We narrowed to 813 results for: TIM

Showing: 621 - 640 of 813 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

  5. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

  7. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

  8. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

  9. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...

  10. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...from producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second edition...

  12. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media ...

  13. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...how you’re decorating your lab for all upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters...

  14. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...person, we selected the finalist based on the first time of submission.)     We love the way the names...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...the repository that have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...welcoming committee. However, the majority of the time was spent with Addgenies meeting each other's friends...

  19. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ... titer, serotype, injection site, and incubation time. Learn more about the Data Hub in this blog post...
Showing: 621 - 640 of 813 results