We narrowed to 818 results for: TIM

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...

...from producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second edition...

...how you’re decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping...

...welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media ...

...how you’re decorating your lab for all upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters...

...person, we selected the finalist based on the first time of submission.)     We love the way the names...

...the repository that have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As...

...conflict with our values. For this reason, our timeline has accelerated. We significantly reduced posting...

...welcoming committee. However, the majority of the time was spent with Addgenies meeting each other's friends...

...Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide share. ...