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We narrowed to 830 results for: ache

Showing: 621 - 640 of 830 results
  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...
  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...is codon optimization? Codon optimization is an approach in gene engineering to improve gene expression... several codons that encode the same amino acid. Each organism, however, may preferentially use one codon...
  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents...found that downregulating expression of miR124a achieved a similar improvement in the forced swim test....
  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... filtered out inflammatory questions, and even coached us on how to write our AMA submission to avoid ...etc. database. Still have questions? I am easy to reach and happy to help. Additional Resources on the ...
  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...cells was being explored and it allowed control over each individual component of the system. However, it ...details on what exactly has been inserted or deleted. Each lab will use a slightly different method to genotype...
  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...because the purified plasmid, cells, and media used in each of the wells are not independent: they were derived...consuming and ultimately more important. Ideally, each biological replicate should use fresh media, test...
  7. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...interpretation of the experiment would require access to each individual video. To PLOS’s credit, the organization...three-fold. Firstly, we rely on many other labs to teach us how to do experiments and understand their data...
  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...however, eukaryotes have muliple polymerases which are each responsible for a specific subset of RNAs. To gain... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...
  9. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... makes it a perfect candidate for introductory teaching and research environments (Idalia & Bernardo, ...scientists around the world worked to characterize each gene’s function. Some key genetics research with...
  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...recombination events, but because these events are rare, each requires selection. These two steps are (Figure...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...
  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...screening platform developed here is also a promising approach for developing sensors for other targets. Figure...
  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...different sets of bands on the gel corresponding to each type of multimer present in your sample. In these...26104708; PMCID: PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with...
  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation...
  14. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ... RNA-seq data. In addition to unique proteins on each cell type, the cell-surface shared proteome of astrocytes...transcription-translation system (Figure 6). This approach eliminates the need for expensive reagents and...
  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the mice ...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...
  16. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers...edge over other graduates during the recession. As each advertised job received many applications, employers...
  17. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces... different tropisms but, as you can see, AAVs of each serotype can transduce multiple tissues. So how ...
Showing: 621 - 640 of 830 results