Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 816 results for: ache

Showing: 621 - 640 of 816 results

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...different sets of bands on the gel corresponding to each type of multimer present in your sample. In these...26104708; PMCID: PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation...

  5. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the mice ...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers...edge over other graduates during the recession. As each advertised job received many applications, employers...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...members really should have been getting feedback from each other. Though meetings are helpful, they are certainly...lines of communication, but if teammates don’t trust each other, none of that matters. If you are on a team...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces... different tropisms but, as you can see, AAVs of each serotype can transduce multiple tissues. So how ...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...Reusing the same bottle of serum again and again for each subculture can enhance the growth of mycoplasmas...Molecular Biology (University of Milan, Italy) and a Bachelor's degree in Biotechnology.       References Degeling...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...target site (Fig. 3B). The INTEGRATE system can achieve ~100% integration of DNA up to 10 kB in size in...in the lab (Sleeping Beauty, piggyBac, and Tol2), each of which has several different applications (mutagenesis...
Showing: 621 - 640 of 816 results